A critical problem associated with establishment of stable transformants of mammalian cells containing drug resistant markers is that many cell types are dependent on cell to cell interaction for growth. Thus, when the transfection efficiency is very low the successfully, but rare transfected drug resistant cells may be inhibited from growing due to low cell density. Moreover, cytotoxicity of t...

knowledge of the stand condition and the gradual replacement of tree species in different development stages and phases provide appropriate information related to the close to nature treatments. stem exclusion phase is one of the important development phases in the forest stands development. the aim of study was to analyze the characteristics of dead trees in the stem exclusion phase in the les...

Large cardiac spheroids (i.e., > 500 µm diameter) that self-induce an ischemic core were generated by co-culture of cardiomyocytes derived from induced pluripotent cells (hiPSC-CMs) and primary fibroblast (hCF). Without modification culture conditions, spheroids’ large size allowed the recreation a dead core, fibrotic remodelling tissue stiffening.

Background: Mass cytometry (Cytometry by Time of Flight, CyTOF) allows single-cell characterization on the basis of specific metal-based cell markers. In addition, other metals in the mass range such as silver can be detected per cell. Bacteria are known to be sensible to silver and a protocol was developed to measure both the number of affected cells per population and the quantities of silver...

After apoptosis or necrosis, macrophages clear dead cells by phagocytosis. Although this process is efficient, circulating nucleosomes can occur in certain diseases, presumably reflecting either increased production or impaired clearance. To investigate the generation of blood nucleosomes, graded numbers of apoptotic and necrotic cells were administered to healthy mice, and levels of blood nucl...

The cellular reactions in tuberculosis form one of the striking features of the disease and it is important to note that they can be produced by the dead bacilli. In 1890, Maffucci (1) studied the effect of subcutaneous injections of dead organisms; he recorded the formation of abscesses and the subsequent death of the animals from marasmus. The next year, Koch (2), in the well-known article de...

The first section of this paper reviews the research on development avascular tumours, which are tumours without a blood artery network to supply their cells with nutrients. process is then illustrated utilising classical PDE model, composed an outer layer actively dividing and inner core dead (referred as necrotic cells) (called proliferating cells). model takes into account both necrosis (a t...

UNLABELLED In patients with moderate to severe high-frequency hearing loss, cochlear damage may include "dead regions" where there are no functional inner hair cells and/or associated neurons. AIM This study examines speech recognition in sensorineural impaired hearing patients with and without cochlear dead regions at high frequencies. METHODS a clinical and experimental study was made of ...

In sensorineural hearing loss, damage to inner hair cells or the auditory nerve may result in dead regions in the cochlea, where the information transmission is disrupted. In cochlear implants, similar dead regions might appear if the spiral ganglia do not function. Shannon et al. [J. Assoc. Res. Otolaryngol. 3, 185-199 (2002)] simulated dead regions of varying size and location using a noiseba...

In Apis mellifera, hygienic behavior involves recognition and removal of sick, damaged or dead brood from capped cells. We investigated whether bees react in the same way to grouped versus isolated damaged capped brood cells. Three colonies of wild-type Africanized honey bees and three colonies of Carniolan honey bees were used for this investigation. Capped worker brood cells aged 12 to 14 day...