Phosphoramide mustard, an active metabolite of cyclophosphamide, has been reacted separately with guanosine and deoxyguanosine in aqueous solution at pH 7.4. The major adduct which was formed in each case has been isolated by reverse-phase high-pressure liquid chromatography. The structure of the major adduct, as determined by a combination of ultraviolet and field desorption mass spectrometry,...

OBJECTIVE To evaluate the dose-response relationship between arsenic (As) exposure and markers of oxidative damage in Bangladeshi adults. METHODS We recruited 378 participants drinking water from wells assigned to five water As exposure categories; the distribution of subjects was as follows: (1) less than 10 μg/L (n=76); (2) 10 to 100 μg/L (n=104); (3) 101 to 200 μg/L (n=86); (4) 201 to 300 ...

Primary mouse embryo cell cultures were grown in the presence of [14C]guanine, labeling primarily deoxyguanosine residues in the cellular DNA, or in the presence of [14C]adenine, labeling both deoxyguanosine and deoxyadenosine residues in the cellular DNA. These cultures were subsequently exposed to 7,12-[3H]dimethylbenz(a)anthracene for 24 hr. The DNA was isolated and hydrolyzed to deoxyribonu...

DOI: 10.1002/jssc.202001081 The cover picture shows a new HPLC strategy for quantitative analysis of multiple components via single marker (QAMS) to determine nine nucleosides (cytidine, uridine, 2'‒deoxyuridine, inosine, guanosine, 2'‒deoxyguanosine, thymidine, adenosine and 2'‒deoxyadenosine) in Rhizoma Paridis the first time. results methodology validation showed that there was no significan...

The influence of 2'-deoxyguanosine (dG) oxidation at the C-8 position on N-glycosidic bond stability was in­ vestigated. A kinetic analysis of dG and 8-oxo-2'-deoxyguanosine (8-oxodG) depurination reactions was carried out in water solutions at pH ranging from 2 to 7.4 and temperature of 100 °C. The results indicate that N-glyco­ sidic bond of 8-oxodG is significantly more stable in comparison ...

【Introduction】 8-Oxo-2 ́-deoxyguanosine (8-oxo-dG) is a representative nucleoside damage that is formed by oxidation of 2 ́-deoxyguanosine (dG) with reactive oxygen species (ROS), and its presence has been linked to aging, cancer, etc [1]. Unlike dG, 8-oxo-dG forms stable base pairs with both 2 ́-deoxycytidine (dC) and 2 ́-deoxyadenosine (dA). Based on the base-pairing properties of 8-oxo-dG, DNA p...

Under homeostatic conditions, an equilibrium state between amounts of free radicals formed and their scavenging is observed. Free radicals are destructive only when present in excess. Pathological changes within cells and tissues can result from a persistent excess of free radicals. Living organisms are increasingly exposed to oxidative stress, resulting in oxidative DNA modifications. One such...

BACKGROUND AND AIM: Cadmium is a toxic heavy metal exerting several adverse effects in humans, especially for kidney, bone, liver, and cardiovascular system. In particular, genotoxic may occur through epigenetic mechanisms, but direct genotoxicity has been suggested. 8-oxo-7,8-dihydro-2’deoxyguanosine (8-oxodG) an oxidized derivative of deoxyguanosine, largely used as biomarker oxidative stress...

Abstract Background Biomarkers have become a valuable tool in environmental assessment, since they contribute to predicting contaminants monitoring programmes. This study aimed investigate the toxicity of heavy metal pollution Lake Qarun using multibiomarker approach (morphological, oxidative stress, genotoxicity, stress proteins) Solea aegyptiaca fish. During winter and summer seasons, water f...