In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviae strains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in Vero an...

conclusions our study emphasized the increasing role of esbl genes, especially blactx-m15 in epec isolates. results of 42 epec, eight isolates carried the blactx-m1. none of the isolates carried blactx-m2 and blactx-m9. the blactx-m15 variant was identified in all of blactx-m1-positive isolates. furthermore, blashv and blatem genes were detected in 40.5% (n = 17) and 19% (n = 8) of all epec iso...

This study designed to isolation virulence E. coli, specific phages and identify in rabbits biochemically, serologically, detect genes using PCR susceptibility antimicrobial range. For propose fifty-fecal-swab samples collected from diarrheic freshly-dead-young-rabbits were presented bacteriological examination. Data showed twenty-seven (54%) gave positive results of Escherichia coli. in-vitro ...

conclusions etec and eaggec were the most detected e. coli among stool and uti samples, emphasizing the need to dedicate more health care attention to this group. in addition, our phylogenetic study may be helpful in figuring out the infection origin and for epidemiological studies. nonetheless, more research studies with larger sample sizes are suggested for confirming our results. results ove...

The inhibitory activities of known microcins were evaluated against some diarrheagenic Escherichia coli strains. Some antibacterial properties of microcin J25, the most active one, were studied. A rapid two-step purification was performed. The MIC and the minimum bactericidal concentration of J25 against E. coli O157:H7 were 1 and 100 microg ml(-1), respectively. A 10(4)-CFU ml(-1) contaminatio...

Heat-stable toxins (STs) produced by enterotoxigenic bacteria cause endemic and traveler's diarrhea by binding to and activating the intestinal receptor guanylyl cyclase C (GC-C). Advances in understanding the biology of GC-C have extended ST from a diarrheagenic peptide to a novel therapeutic agent. Here, we summarize the physiological and pathophysiological role of GC-C in fluid-electrolyte r...

Diarrhea caused by multidrug-resistant bacteria is an important public health problem among children in developing countries. The prevalence and antimicrobial susceptibility of diarrheagenic Escherichia coli in 346 children under 5 years of age in Ifakara, Tanzania, were studied. Thirty-eight percent of the cases of diarrhea were due to multiresistant enterotoxigenic E. coli, enteroaggregative ...