Chromatography on methylated albumin-kieselguhr of RNA from Saccharomyces cerevisiae was used to separate stable RNA from a tenaciously bound DNA-like RNA fraction. The tenaciously bound RNA, which was eluted with a dilute solution of sodium dodecyl sulphate, was characterized as messenger-like RNA by its sedimentation behaviour, nucleotide composition, lack of methylated bases and labelling ki...

The determination of the starch in sugar crystal is not a simple problem, as it is associated with closely related carbohydrates that affect its separation. The coloration of sugar itself if not under proper adjustment would affect usually the accuracy in the later photometric examination of the starch iodine complex formed. Spectrophotometric methods conventioilally used for this purpose thoug...

A study was made of the nuclear RNA in HeLa cells with particular reference to the rapidly labelled fractions. It was found that if cells were incubated at a high density, that is, under 'step-down' conditions, there was a rapid accumulation of RNA in the nucleus. The fraction of the nuclear RNA which includes rapidly labelled RNA and which binds tightly to columns of methylated albumin on kies...