Oxidative stress (OS) has been implicated in various degenerative diseases in aging. In an attempt to quantify OS in a cell model, we examined OS induced by incubating for 30 min with various free radical generators in PC12 cells by using the dichlorofluorescein (DCF) assay, modified for use by a fluorescent microplate reader. The nonfluorescent fluorescin derivatives (dichlorofluorescin, DCFH)...

The natural polyphenol curcumin and its metal coordinated complexes show obvious benefits in the medical therapies of cancer and several neurodegenerative diseases. On the other side their stability and bioavailability are critical issues. The present study is an attempt to address the stability and functionality of curcumin upon complexation with transition metal ions. We have synthesized and ...

objective(s):  the role of the apoptosis repressor with caspase recruitment domain (arc) in apoptosis and in certain hypertrophic responses has been previously investigated, but its regulation of endothelin-1 induced cardiac hypertrophy remains unknown. the present study discusses the inhibitory role of arc against endothelin–induced hypertrophy. results:in present study endothelin treated card...

introduction: d-galactose (d-gal) is well known as an appropriate agent to induced aging effects in the in vivo and in vitro models. in the present study, we selected crocin, the main constituent of crocus sativus l. (saffron), against d-gal cytotoxicity in human neuroblastoma sh-sy5y cells. materials and methods:  cells were pretreated with crocin (25-500 µm) for 24 h and then exposed to d-gal...

Since distributed coordination function (DCF) is the basis protocol for IEEE 802.11 standard wireless local area networks, many modifications have been proposed in the literature to improve its throughput performance particularly under congested environments. These improvements are achieved by modifying the contention window reset mechanism in which a DCF station immediately reduces the window ...

Cytotoxicity by unconjugated bilirubin involves disturbances of membrane structure, excitotoxicity and cell death. These events were reported to trigger elevated free radicals production and impairment of calcium homeostasis, and to result in loss of cell membrane integrity. Therefore, this study was designed to investigate whether interaction of clinically relevant concentrations of free uncon...

Discriminative Correlation Filters (DCF) are efficient in visual tracking but suffer from unwanted boundary effects. Spatially Regularized DCF (SRDCF) has been suggested to resolve this issue by enforcing spatial penalty on DCF coefficients, which, inevitably, improves the tracking performance at the price of increasing complexity. To tackle online updating, SRDCF formulates its model on multip...

Experiments were conducted in a dynamic chamber system to measure the formation of secondary organic aerosol (SOA) and particle-bound reactive oxygen species (ROS) produced from limonene ozonolysis in the presence of NO by varying the ratio of O3 to NO. A diffusion cell system was used to produce the constant input of limonene in to the chamber, and six sets of experiments were conducted. The c...

background: cryopreservation of pre-antral follicles is a hopeful technique to preserve female fertility. the aim of the present study was to evaluate reactive oxygen species (ros) and total antioxidant capacity (tac) levels of mouse vitrified pre-antral follicles in the presence of alpha lipoic acid (ala). methods: isolated pre-antral follicles (140–150 µm in diameter) were divided into vitrif...

It has been well appreciated that aldosterone (Aldo) plays a direct profibrotic role in the kidney but the underlying mechanism is unclear. We examined the role of Aldo in epithelial-mesenchymal transition (EMT) both in vitro and in vivo. Exposure of human renal proximal tubular cells to Aldo for 48 h dose dependently induced EMT as evidenced by conversion to the spindle-like morphology, loss o...