We present a new digital two-step reconstruction method for off-axis holograms recorded on a CCD camera. First, we retrieve the complex object wave in the acquisition plane from the hologram's samples. In a second step, if required, we propagate the wave front by using a digital Fresnel transform to achieve proper focus. This algorithm is sufficiently general to be applied to sophisticated opti...

Examination of chromogenically stained tissues has been the hallmark of clinical diagnostic pathology and cancer research for nearly a century. Fixed and stained tissue on microscope slides has been the routinely used method of data storage. Pathological samples to be viewed were routinely fixed and embedded in paraffin for long term storage. Thin slices were then made from the paraffin samples...

A scanning magnetoresistive (MR) microscope is developed for high-resolution imaging of magnetic tapes and digital media. Second-harmonic detection is used to remove thermal anomalies. We are able to image sufficient lengths of tape for authentication purposes and for data recovery from damaged samples. The second-harmonic technique provides high-contrast magnetic images of the magnetic storage...

We introduce the use of a birefringent crystal with lensless digital holography to create an on-chip differential interference contrast (DIC) microscope. Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the ...

This paper presents a new criterion for blob detection applied in the framework of scale-space based segmentation of objects from digital medical images. The proposed method is based on fitting the blob to a standard shape, such as the Gaussian, subject to constraints based on the blob support area or the total variation. The method has been verified and compared with the conventional procedure...

Abstract. The configuration of new survey approaches for digitizing tiny artefacts is gradually emerging in favour the related results sharing modalities such as 3D printing and or Cultural Heritage open-access datasets. Unfortunately, due to still high cost instrumentations time required specialized operators training, both digital documentation small dissemination data are a long way from bec...

Membrane-binding of a human blood clotting protein, factor VIII (FVIII), was studied by cryoelectron microscopy. Well visible gap junctions between adjacent phosphatydyl serine containing phospholipid vesicles were observed upon FVIII association. Digital images acquired with a JEOL2010FEG transmission electron microscope equipped with a 4x4k CCD camera were analysed with the Digital Micrograph...