• Many investigations of the cardiovascular system require estimations of regional or single vessel blood flow. Although direct methods ' have been devised for this purpose, most have a limited application in man because they involve exposure of the appropriate blood vessel. When multiple and frequent flow estimations over a long period are desired in experimental animals, repeated exposures of...

A radioimmunoassay (RIA) for human pro-LRF(14-69)OH was developed with an antiserum, generated in a rabbit, to [Tyr67]pro-LRF(47-67)NH2 conjugated to BSA. This antiserum bound 28-32% of [125I]pro-LRF(14-69)OH at a final dilution of 1:2500 and the binding was inhibited by pro-LRF(14-69)OH in a dose-dependent manner. The sensitivity of the RIA was 31.2-62.5 pg and the dose that inhibited 50% of t...

This report presents a new, simple and reliable method of quantitating left-to-right shunts from arterial dilution curves. When indicator (e.g., indocyanine green) is injected centrally (right ventricle or pulmonary artery) and blood is withdrawn rapidly from either the aorta or other central systemic artery, in the presence of a left-to-right shunt the recorded indicator-dilution curve usually...

New technologies that utilize capillary-based immunoassays promise faster and more quantitative protein assessment compared to traditional immunoassays. However, similar to other antibody-based protein assays, optimization of capillary-based immunoassay parameters, such as protein concentration, antibody dilution, and exposure time is an important prerequisite to the generation of meaningful an...

The azo dye Coomassie Blue is relatively non-toxic, has a rapid disappearance rate, and does not cause any skin discoloration (Taylor and Shillingford, 1959). Because it has these properties the dye can be given repeatedly to man at short intervals of time, a particularly important quality for investigations based upon the Stewart-Hamilton indicator dilution curve. Indeed, the dye has already b...

We developed a precise and accurate graphite furnace atomic absorption spectrometric method for the direct determination of zinc in serum. Serum samples are analyzed after 20-fold dilution with water of ultrapure analytical grade. No other reagent is used, from the moment of sampling until measurement. During atomization, the argon flow is kept at 150 mL/min instead of gas stop, to decrease the...

The assay for C-reactive protein has been fully automated as a particle-counting immunoassay. For cerebrospinal fluid, cord serum, and adults' serum its range of sensitivity extends from 1 microgram/L to 300 mg/L, with a minimal sample dilution of twofold and a maximal dilution of 50-fold being required. This range is so broad because free antibodies are added to the reaction medium. However, w...

• Many investigations of the cardiovascular system require estimations of regional or single vessel blood flow. Although direct methods ' have been devised for this purpose, most have a limited application in man because they involve exposure of the appropriate blood vessel. When multiple and frequent flow estimations over a long period are desired in experimental animals, repeated exposures of...

This assay for oxalate in serum or plasma, by a radioenzymatic isotope dilution technique, is sensitive, specific, and accurate. The oxalate in 5 mL of serum or plasma ultrafiltrate is precipitated with Ca2, dissolved in acid, extracted with ether, and the extract evaporated under nitrogen and redissolved in citrate buffer. The oxalate in this solution is decarboxylated with oxalate decarboxyla...

A competitive enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody (M898) was developed for detection of bovine antibodies to Leptospira interrogans serovar pomona. This assay was evaluated using field sera (n = 190) with serovar pomona microscopic agglutination test (MAT) titers of > or =100 as the positive population (group A); field sera (n = 1,445) which were negat...