Copper(II)  complex  [Cu(dmbt)2(H2O)](ClO4)2 (1)  was  prepared  from  the  reaction  of  copper(II) perchlorate  hexahydrate  with  2,2'-dimethyl-4,4'-bithiazole  (dmbt)  ligand  in  methanol  at  ambient temperature. The complex was quantitatively and qualitatively characterized by elemental analysis, absorption  and  infrared  spectrometries.  Complex  [Cu(DMSO)5](ClO4)2 (2)  was  also  synt...

Stereospecifically cyclopropanated sugar deriva­ tives are not only interesting from their biological point of view, but they can also serve as templates for the synthesis of natural products using the chiron approach [1]. The previous successes in the synthesis of a number of cyclopropanated sugar derivatives via diplacement of the triflyl group [2] promoted us to investigate a similar reactio...

The dorC gene of the dimethyl sulfoxide respiratory (dor) operon of Rhodobacter capsulatus encodes a pentaheme c-type cytochrome that is involved in electron transfer from ubiquinol to periplasmic dimethyl sulfoxide reductase. DorC was expressed as a C-terminal fusion to an 8-amino acid FLAG epitope and was purified from detergent-solubilized membranes by ion exchange chromatography and immunoa...

Mouse erythroleukemia (MEL) cells contained galactosyltransferase (GT), fucosyltransferase (FT), and A/-acetylglucosaminyltransferase activities and only a small amount of sialyltransferase activity. Dimethyl sulfoxide-treated MEL cells, un dergoing erythrodifferentiation, contained twice as much GT, 20 to 50% less FT, and approximately the same amount of Nacetylglucosaminyltransferase activiti...

In order to determine if cyclic adenosine 3':5'-monophosphate(cyclic AMP)-dependent protein kinase has a role in the expression of chemically induced differentiation of HL-60 cells, levels and subcellular distribution of this enzyme were studied during this process. Cyclic AMP binding protein and stimulated kinase activities increased moderately in cytosol and more mark edly in nucleosol and no...

The lipopolysaccharide (LPS) of Chromatium vinosum has anticomplementary activity. This anticomplementary activity is destroyed by alkaline digestion of the LPS and is suppressed by both Mg2+ and Ca2+ ions. Treatment of the LPS with ethylenediaminetetraacetic acid, sodium deoxycholate, or dimethyl sulfoxide did not affect its toxicity toward mice; however, alkaline-treated LPS was not toxic. Tr...

Both peripheral blood monocytes and neutrophils are known to be capable of lysing a variety of extracellular tumor and non-tumor cell targets. The HL-60 human promyelocytic leukemia cell line has served as a useful model of human granulocyte and macrophage differentiation in studies from many laboratories. We have previously reported that phorbol diesters, which induce differentiation along the...

In order to determine if cyclic adenosine 3':5'-monophosphate- (cyclic AMP)-dependent protein kinase has a role in the expression of chemically induced differentiation of HL-60 cells, levels and subcellular distribution of this enzyme were studied during this process. Cyclic AMP binding protein and stimulated kinase activities increased moderately in cytosol and more markedly in nucleosol and n...