OBJECTIVES To determine the antimicrobial susceptibility of Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pneumoniae causing community-acquired lower respiratory tract infection in the UK during 2002/2003 and to compare susceptibilities determined locally by disc diffusion with agar dilution MICs determined at a central laboratory. METHODS H. influenzae, M. catarrhalis and S...

BACKGROUND & OBJECTIVE Conventional methods to detect methicillin resistance in Staphylococcus aureus are inadequate as expression of resistance is subject to environmental and conditional expression of PBP2a antigen. The objective of the present study was to determine methicillin resistance in S. aureus by conventional susceptibility (oxacillin disc diffusion and oxacillin MIC) and molecular m...

The aim of this study was to characterize the phytochemicals and investigate antimicrobial efficacy shade dried roots Asparagus adscendens. powder were extracted with chloroform after removal solvent under reduced pressure subjected for chromatographic separation. After column chromatography purification five compounds i.e .hexacosanyltriacontanoate, hexatriacontanone, 2,3,5,7-tetramethoxydi hy...

The boundary layer of the air flow blowing surfaces brake mechanism prevents heat transfer from heated surfaces. obtained analytical dependences velocities` and temperatures` distribution in represent field as a multilayer structural model, laws change velocities temperatures depend on various sub layers layer. According to these dependencies, thermal model “ventilated disc - external condition...

The emergence of Methicillin-resistant Staphylococcus aureus (MRSA) has posed a serious therapeutic challenge. We report the prevalence and antibiotic susceptibility pattern of MRSA in the hospitalized patients in a rural tertiary care hospital in India. The study comprised of 97 Staphylococcus aureus isolated from a total of 400 clinical samples obtained from hospitalized patients. Antibiotic ...

The disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and gradient-plate techniques were implemented to evaluate the antibacterial activity of honey and propolis against Staphylococcus aureus and Escherichia coli. The growth of S. aureus was inhibited by application of propolis and honey at concentrations of 2.74 to 5.48 mg ml and 375.0 at m...

Studies in the 1960s demonstrated the problems of variability in susceptibility testing methods, especially those affecting the performance of disc diffusion procedures. These studies made apparent the need for standardization and resulted in more clearly defined performance limits for growth medium, incubation conditions, inoculum concentration, disc content for diffusion methods, the setting ...

The susceptibility of two collections of coagulase-negative staphylococci (CNS) isolated from clinical specimens for teicoplanin and vancomycin were compared. They comprised 91 and 101 isolates, collected in 1985 and 1994 respectively, from different departments of a teaching hospital. MICs of vancomycin and teicoplanin were determined by a modified Etest method. Additionally, a disc diffusion ...

The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S. aureus i...