for a long time, scientists have tried to describe disorders just by genetic or environmental factors. however, the role of epigenetics in human diseases has been considered from a half of century ago. in the last decade, this subject has attracted many interests, especially in complicated disorders such as behavior plasticity, memory, cancer, autoimmune disease, and addiction as well as neurod...

Background: It is well documented that fetal DNA can cross the placenta and is present in peripheral maternal blood during pregnancy in human. This fetal DNA also named circulating cell free fetal DNA, has emerged as a valuable source for genetic evaluation. Compared with humans, ovine species have a different structure of placental (synepitheliochorial) with no direct contact between the troph...

background: indirect genetic diagnosis using polymorphic dna markers can detect carriers of hemophilia a. this technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. in the present study, we examined usefulness of intragenic marker bcl i restriction fragment length polymorphism (rflp) at intron 18, for carrier detec...

Background: Indirect genetic diagnosis using polymorphic DNA markers can detect carriers of hemophilia A. This technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. In the present study, we examined usefulness of intragenic marker BclI restriction fragment length polymorphism (RFLP) at intron 18, for carrier detect...

Background: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. Methods: ...

Introduction: tegrity is the most important component for transferring genetic information Sperm DNA fragmentation is considered a component of male infertility. Its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. In this study, we investigate the relationship between sperm parameters of motility, morphology, concentratio...

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...