The interactions of host and infecting strain in ovine transmissible spongiform encephalopathies are known to be complex, and have a profound effect on the resulting phenotype of disease. In contrast to classical scrapie, the pathology in naturally-occurring cases of atypical scrapie appears more consistent, regardless of genotype, and is preserved on transmission within sheep homologous for th...

Cloning of cells from peri-implantation embryos by blastocyst injection was used to investigate the time of X-chromosome inactivation in that part of the ectoderm lineage giving rise to foetal tissues of the mouse. Matings were arranged so that the two X-chromosomes of female donor cells controlled two distinct coat colours and host blastocysts were of a third colour genotype. No coat chimaeras...

BACKGROUND Donor cell engraftment is critical for the success of allogeneic bone marrow transplants. Graft failure is a result of donor cells either failing to engraft initially or being eliminated at later time points. Donor cell engraftment is facilitated by donor T cells, which eliminate residual host hemato-lymphoid effector cells such as NK cells and T cells. METHODS We aimed to explore ...

Surface-localized rat RT1 alloantigens on isolated hepatocytes have been used to achieve partial purification of putative premalignant liver cells from rats undergoing chemically induced hepatocarcinogenesis. Genotypic mosaic rat livers were con structed by transplantation of carcinogen-altered F-344 (n77'"1) or WF (RT1U)donor liver cells into livers of WF x F-344 F, host rats, whose liver cell...

A patient with acute leukemia and her family including four HLA-identical siblings were analyzed to select a donor who was not only HLA- but also minor histocompatibility (mH) antigen compatible for allogeneic bone marrow transplantation (BMT). The HLA-A2 restricted mH antigen-specific HA-1, -2, -4, and -5 cytotoxic T-lymphocyte (CTL) clones were used to type the family members for expression o...

The recent paper by Arpinati et al1 elegantly demonstrates that the numbers of T helper 2–inducing dendritic cells (pre-DC2s) are increased in granulocyte colony-stimulating factor (G-CSF)– mobilized allografts. Pre-DC2s on subsequent activation lead to polarization of naive CD4 T cells toward a Th2 phenotype. In the accompanying Focus on Hematology article, Liu and Blom2 suggest that acute gra...