Confocal fluorescence correlation spectroscopy as a time-averaging fluctuation analysis combining maximum sensitivity with high statistical confidence has proved to be a very versatile and powerful tool for detection and temporal investigation of biomolecules at ultralow concentrations on surfaces, in solutions, and in living cells. To probe the interaction of different molecular species for a ...

A novel approach to design Tunable Dual-Band Band-Stop filters will be presented in this paper. These filters have a new configuration with a coplanar microstrip line loaded with SteppedImpedance Resonators (SIRs). These can be tuned by using tuning elements such as a tuning diode and ferroelectric capacitor. The Dual-Band Band-Stop Filter (DBBSF) and Dual-Band Band-Pass Filter (DBBPF) have bec...

background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...

in this article, the linear array (with 20 elements in the horizontal orientation) of microstrip antenna is examined with horizontal polarization. the set includes three microstrip substrates. there is a power network in two lower substrates and microstrip patches in the upper substrate. excitation of patches has been done as coupled through the power network in two lower substrates. using the ...

Additive manufacturing (AM) can revolutionise engineering by taking advantage of unconstrained design and overcoming the limitations traditional capabilities. A promising application AM is in catalyst substrate manufacturing, aimed at enhancement catalytic efficiency reduction volume weight reactors exhaust gas aftertreatment systems. This work addresses fabrication innovative, hybrid monolithi...

The aluminum cold spray feedstock powder was single- and dual-reinforced with no greater than 2 vol% boron nitride nanoplatelets (BNNP) and/or nanometric carbide (nB4C). These powders were sprayed onto Al-6061 substrates then heat-treated in an argon environment. In addition, micro- nano-indentation hardness wear testing performed on the samples. Further microscopy optical profilometry used to ...

Artificial metalloenzymes result from anchoring an organometallic catalyst within evolvable protein scaffold. Thanks to its dimer of dimers quaternary structure, streptavidin allows the precise positioning two metal cofactors activate a single substrate, thus expanding reaction scope accessible artificial metalloenzymes. To validate this concept, we report herein on our efforts engineer and evo...

Metal–organic framework (MOF)‐derived carbon composites have been considered as the promising materials for energy storage. However, construction of MOF‐based with highly controllable mode via liquid–liquid synthesis method has a great challenge because simultaneous heterogeneous nucleation on substrates and self‐nucleation individual MOF nanocrystals in liquid phase. Herein, we report bidirect...

Using a newly developed near-infrared (NIR) dye that fluoresces at two different wavelengths (dichromic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kinase B, and a method to quantitatively report this enzyme's activity in real time. Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF ...

A method for fabricating planar implantable microelectrode arrays was demonstrated using a process that relied on ultra-thin silicon substrates, which ranged in thickness from 25 to 50 μm. The challenge of handling these fragile materials was met via a temporary substrate support mechanism. In order to compensate for putative electrical shielding of extracellular neuronal fields, separately add...