The total cellular content of the fluorescent mitochondrialspecific dye rhodamine 123 (Rh-123) was quantified by butanol extraction as a function of time of exposure and dose for a variety of cell lines. These results were compared with observa tions made by fluorescence microscopy on dye localization and mitochondrial morphology. There appeared to be two categories of cell types based on Rh-12...

The total cellular content of the fluorescent mitochondrialspecific dye rhodamine 123 (Rh-123) was quantified by butanol extraction as a function of time of exposure and dose for a variety of cell lines. These results were compared with observa tions made by fluorescence microscopy on dye localization and mitochondrial morphology. There appeared to be two categories of cell types based on Rh-12...

India has a very rich diversity and plantkingdom is certainly a treasure house of diverse natural products. One such product from nature is the dye. In this study, natural colorants mainly flavonoids and carotenoids present in the Marigold flower (TageteserectaL.) were extracted using Soxhlet extraction method and other conventional techniques under different operating conditions. Spectrophotom...

This work presents the valorization of Millettia laurentii carpentry sawdust waste into sustainable natural dye for ecological dyeing and functional finishing wool textiles. A simple aqueous extraction method was used to isolate dye, then extracted bio-dye subjected different qualitative quantitative analyses. Wool yarns were dyed in conjunction with metal salts (aluminum sulfate, ferrous coppe...

Sudan dyes I, II, III & IV are synthetic, oil-soluble, red-coloured azo dyes. These suspected genotoxic carcinogens and have been used in the adulteration of palm oil products. Many experimental studies on animal specimen confirmed formation tumour due to presence different food In this study, a Simple Thin Layer Chromatographic method is described for qualitative detection Sudan-I, gotten ...

The photochemical effects of near-UV light on chromatin labeled with the vital DNA dye Hoechst 33342 (H33342) are studied. Several types of experiments demonstrate that illumination at both 365 and 410 nm results in significant cross-linking of proteins with the DNA. Fluorescence microscopy of dye-stained Xenopus XTC-2 nuclei shows that UV illumination has effects similar to chemical fixation b...

This article reports on a method to determine synthetic dyes in yogurts and milk drinks. Initially a method for extraction of artificial dyes was developed to pretreat samples in order to extract most of the artificial colorants. Then, the colorants were analyzed by HPLC-PAD using gradient elutions. The method was linear in the range of 0.5-25mgL(-1) colorants (0.9991<R(2)<0.9998), with LOD of ...

cDNA Sequencing To generate cDNA, RNA was isolated from frozen tissue with the RNeasy minikit (Qiagen), treated with DNase I to degrade residual DNA and complementary DNA was reverse transcribed in vitro using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Where required, normal and polyp tissue was microdissected with the laser capture PALM system. DNA was extracted usi...

Madder (Rubia tinctorum L.) has been used as a dye for over 2000 years with alizarin and purpurin the major natural dyes analysed from extractions undertaken. The use of ethanol as the solvent in the extraction process produced an extract that yielded four anthraquinone compounds lucidin primeveroside, ruberythric acid, alizarin and lucidin-ω-ethyl ether. Gravitational separation of the extract...