Sugar beet (Beta vulgaris L.) leaf plasma membrane vesicles were loaded with an NADH-generating system (or with ascorbate) and were tested spectrophotometrically for their ability to reduce external, membrane-impermeable electron acceptors. Either alcohol dehydrogenase plus NAD(+) or 100 millimolar ascorbate was included in the homogenization medium, and right-side-out (apoplastic side-out) pla...

The present communication is devoted to the description of the isolation, properties, and composition of a particulate unit which we shall refer to as the succinic dehydrogenase complex (SDC). The working hypothesis underlying this and subsequent communications in this series is that SDC is a unit of enzymatic action in the same sense as any well defined soluble enzyme. Since the discovery of s...

A maternal high-fat (HF) diet during pregnancy can lead to metabolic compromise, such as insulin resistance in adult offspring. Skeletal muscle mitochondrial dysfunction is one mechanism contributing to metabolic impairments in insulin resistant states. Therefore, the present study aimed to investigate whether mitochondrial dysfunction is evident in metabolically compromised offspring born to H...

Molybdenum is very toxic to agricultural animals. Mo-reducing bacterium can be used to immobilize soluble molybdenum to insoluble forms, reducing its toxicity in the process. In this work the isolation of a novel molybdate-reducing Gram positive bacterium tentatively identified as Bacillus sp. strain A.rzi from a metal-contaminated soil is reported. The cellular reduction of molybdate to molybd...

Mesozooplankton consumes an important fraction of particulate organic matter sinking down the water column in all oceans. We investigated the vertical distribution (down to 2000 m depth) and diel variability (during a 24 h cycle) of the mesozooplankton carbon requirement (μg C g−1 d−1), estimated by measuring the activity of the electron transport system (ETS), at a fixed station in the open so...

Wosilait and Nason (1) demonstrated that quinones could serve as electron acceptors for the enzymatic oxidation of reduced pyridine nucleotides, and their survey of plant, animal, and microorganism extracts (2) revealed the general occurrence of reduced pyridine nucleotide-quinone reductase activity. This finding and Dam’s (3) earlier report, which indicated that vitamin K was a common constitu...

Transcriptional regulation of the prolactin gene in the decidua differs from that in the pituitary. Several lines of evidence strongly suggest this difference is due to regulation of the prolactin gene in the decidua and other extra-pituitary tissues by tissue-specific transcription factors, which activate distinct promoters to induce prolactin gene expression in extra-pituitary sites compared ...

The energy-dependent uptake of calcium by inverted membrane vesicles of Escherichia coli was investigated. Methods for preparation and storage of the vesicles were devised to allow for the maximal activity and stability of the calcium transport system. The pH and temperature optima for the reaction were observed to occur at pH 8.0 AND 30 DEGREES, RESPECTIVELY. The eft was found that the extent ...

Staphylococcus aureus formed an electron transport system when exponentially growing cells were aerated. Formation of the electron transport system occurred concomitantly with increases in the phospholipids and the carotenoids. The addition of piperonyl butoxide or benzo(a)pyrene at the onset of aeration (i) slowed the formation of the electron transport system, (ii) both inhibited cytochrome o...