Bovine serum albumin undergoes two electrophoretically distinguishable types of interaction in acidic media (1). The first (l-6), which is also shown by ovalbumin, bovine y-pseudoglobulin and oxidized ribonuclease, is observed only in media containing acetate buffer (NaAc-HAc) or other carboxylic acid buffers. The various peaks shown by the electrophoretic patterns obtained in these media corre...

Much evidence suggests that granulocytes are a major source of serum vitamin B12-binding protein (BBP). The latter has three components: a-iglobulin BBP (Transcobalamin I, TC I), fl-globulin BBP (TC II), and a recently described third BBP. Granulocytic BBP has appeared to be identical to TC I except in electrophoretic mobility. In the present study, the dominant BBP of leukocyte extracts from s...

Ribonucleoprotein particles were prepared by treatment of rat liver microsomes with detergents and high concentrations of KCl. They were active in incorporating (14)C amino acids into protein when incubated with cell sap together with ATP, GTP, and a system to regenerate the triphosphates. The albumin of the incubation mixture, soluble at 105,000 g, and that of the fraction released by ultrason...

Paper-strip electrophoretic studies using tris(hydroxymethyl)aminomethane-ethylene diaminetetraacetic acid-boric acid buffer were carried out on the serum of rats bearing primary methylcholanthrene-induced fibrosarcomas as well as 1st and 2nd transplant generation autografts and isografts of such tumors. It was found that, though bearing histologically similar tumors, serum of different rats pr...