BACKGROUND Transcobalamin is essential for the cellular internalization of cobalamin. Methods to quantify the unsaturated protein are available, but few attempts have been made to develop methods to quantify the sum of unsaturated and cobalamin saturated transcobalamin. METHODS gamma-Globulins from two polyclonal rabbit antibodies against recombinant human transcobalamin were used as capture ...

In this article, we describe the kinetic ELISA of Blue Tongue and Epizootic Hemorrhagic Disease viral antibodies in microfluidic channels by monitoring the rate of generation of the enzyme reaction product under static conditions. It has been shown that this format of the immunoassay allows very reliable quantitation of the target species using inexpensive glass microchips and a standard epiflu...

Uveitis and primary intraocular lymphoma (PIOL) are diseases associated with the invasion of lymphocytes into various regions of the eye, accompanied by expression of inflammatory cytokines. While these diseases are very different in terms of survivability and treatment options they have similar symptoms that make accurate diagnosis challenging. Furthermore, the diagnostic yield with state-of-t...

for the evaluation of autoantibodies in pemphigus vulgaris (pv), an indirect elisa assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (m.w.) of 130-160 kda. the evaluation of pemphigus igg, igm and iga autoantibodies in 75 patients and 50 control sera indicated that this indirect elisa assay was a useful method for the detection of ...

background: patients with prolonged neutropenia and/or severe underlying immunosuppression are at thegreatest risk for disseminated aspergillosis. this study was undertaken to determine the incidence of invasiveaspergillosis by platelia aspergillus enzyme-linked immunosorbent assay elisa kit in high risk children admitted tothe hematology ward of dr. faghihi hospital, shiraz university of medic...