The ELISA and an immunoblotting technique were used to study F38-type mycoplasmas - an important cause of contagious caprine pleuropneumonia - and a number of related mycoplasma species, subspecies, types or serogroups. Two-way ELISA cross-reactivity was demonstrated between five mycoplasmas, namely strain F38, Mycoplasma mycoides subsp. mycoides (LC strain), M. equigenitalium, M. primatum and ...

Acute diarrheal illness is a serious public health issue in developing and developed economies, with high morbidity mortality rates. Children below five years are the most vulnerable, one every six dying from diarrhea. Diarrhea may be caused by various viruses, protozoa, bacteria. Rotavirus, particular, often associated diarrhea children. Early detection of rotavirus infection reduces need for ...

In the present communication, we report on exploitation of a Bloch surface wave-enhanced fluorescence scheme for detection Interleukin (IL)-10 in protein-rich buffer mimicking biological sample. IL-10 is cytokine known its potent anti-inflammatory and immunosuppressive effects. It considered valuable biomarker prognostic prediction both solid tumors hematological malignancies, recently, disting...

Lipopolysaccharide (LPS) is a major surface component of most Gram-negative bacteria and recognized by immune cells as pathogen-associated molecules. Antibodies against O antigens can provide protection infection vaccines have been developed conjugating to carrier proteins enhance immunogenicity. This study aims produce antigen from Escherichia coli analyze the in vivo test results poultry usin...

Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-et...

Genetic alterations in the rpoB gene were characterized in 50 rifampin-resistant (Rif(r)) clinical isolates of Mycobacterium tuberculosis complex from Spain. A rapid PCR-enzyme-linked immunosorbent assay (ELISA) technique for the identification of rpoB mutations was evaluated with isolates of the M. tuberculosis complex and clinical specimens from tuberculosis patients that were positive for ac...

BACKGROUND The cause of past plague pandemics was controversial but several research teams used PCR techniques and dental pulp as the primary material to reveal that they were caused by Yersinia pestis. However, the degradation of DNA limits the ability to detect ancient infections. METHODS We used for the first time immuno-PCR to detect Yersinia pestis antigens; it can detect protein concent...

J. Esterhuysen1 andJ. Barrat2 The reference method to determine the antibody level of a serum sample is seroneutralisation. The principle of this technique is to incubate a constant known amount of rabies virus with a constant volume of the serum to be tested. The virus that has not been neutralised by the antibodies is quantified using an inoculation test either in vivo or in vitro. This metho...

BACKGROUND Adipose tissue produces a number of inflammatory mediators. Circulating concentrations of these inflammatory markers are increasingly used as markers of local or systemic inflammation. This study compares results for 3 inflammatory adipokines measured using 2 techniques, (multiplex and ELISA), and determines the relationships with C-reactive protein (CRP), obesity, and the impact of ...

Detection of circulating galactofuranose (galf) antigens, including galactomannan (GM), by the Platelia Aspergillus (PA) enzyme-linked immunosorbent assay (ELISA) is an important tool in the early diagnosis of invasive aspergillosis (IA). We used a modified pretreatment technique (MT) on consecutive negative PA ELISA plasma samples from IA patients in order to improve the detection of the funga...