The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability produce entire plants without the need for gamete fusion. While embryo proliferation typically performed on solid media, this approach less efficient than liquid which both more cost-effective can lead better results. Thus, study aimed determine most effective combination ...

A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that development from rapid starting one day after culture with an rate 20 to 25% higher than those soaked embryos. In addition, the mean growth developed 60 70% cultured This study also demonstrated a frequency green sp...

An efficient method for in vitro micro propagation and genetic transformation of plants are crucial for both basic and applied research. Maize is one of the most important cereal crops around the world. Regeneration from immature embryo is hampered due to its unavailability round the year. On the contrary mature embryo especially tropical maize is recalcitrant toward tissue culture. Here we rep...

somatic embryogenesis was initiated in c. sativus l. from shoot meristems on ls medium containing bap (2×10-5 m)+naa (2×10-5 m). various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. matured embryos coul...

The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the ...

In human IVF, the concept of 'low responders' is well known and generally defined as women with poor-response to gonadotrophin stimulation in a previous induction cycle. The objective of this retrospective study is to describe and characterise the concepts of 'low-, medium-, and high-response' and 'low, medium, and high responders' in bovine-assisted reproduction by analysing the OPU-IVF result...

The mechanism for induction of tumors by estrogens is still unresolved. Neoplastic transformation of Syrian hamster embryo fibroblasts by diethylstilbestrol (DES) suggests that established principles of chemical carcinogenesis may be involved. The Syrian hamster embryo fibroblast cells provide a system in which now the question can be asked whether the metabolic activation of DES is a prerequis...

The application of tissue culture techniques, particularly in the area of embryo rescue, has had a major impact on the maintenance and development of hybrid embryo from wide crosses. Embryo rescue techniques are directed towards obtaining more efficient survival of embryos in situations where very immature embryos are to be cultured. An in vitro embryo rescue culture technique has been develope...

Callus cultures from root explants of Lathyrus sativus L. ‘Derek’ were tested for their morphogenic capacity. Primary explants (fragments of roots) were cultivated on three induction media. We obtained three lines of callus tissue among which we identified two non-embryogenic lines and one embryogenic line. Callus originally cultivated on modified MS medium supplemented with 0.05 mgL picloram, ...

mouse embryos into blastocysts in vitro have been closely examined (Whitten, 1956, 1957; Brinster, 1965a, b, c), and it has been shown that the requirements depend on the stage of development when cultivation begins (Brinster & Thomson, 1966; Biggers, Whittingham & Donahue, 1967). One of the inter¬ esting findings has been that there is no absolute requirement for the essential amino acids eith...