Callus cultures from root explants of Lathyrus sativus L. ‘Derek’ were tested for their morphogenic capacity. Primary explants (fragments of roots) were cultivated on three induction media. We obtained three lines of callus tissue among which we identified two non-embryogenic lines and one embryogenic line. Callus originally cultivated on modified MS medium supplemented with 0.05 mgL picloram, ...

Factors influencing Agrobacterium-mediated transformation of 15 Vitis rotundifolia, Vitis rupestris, Vitis vinifera and Vitis hybrids were explored. A green fluorescent protein/neomycin phosphotransferase II (GFP/NPT II) fusion gene was used to measure transient and stable transgene expression levels. Embryogenic cell cultures at different developmental stages were co-cultivated with Agrobacter...

genus onobrychis is an important forage crop consisting of approximately 130 annual and perennial species. the present research focused on the cytological studies of embryogenic (e) and non-embryogenic (ne) callus as well as root meristem cells of in vitro and in vivo grown plants. on the contrary to the previous studies, a diploid viciifolia variety with 2n= 2x= 16 chromosomes was observed for...

Abstract Somatic embryogenesis, as a promising biotechnological tool for many conifer trees, has never been applied the Abies nebrodensis species. Although all encouraging results previously obtained by EU LIFE (European program) funded projects in over ten years, critically endangered Sicilian fir remains alarmingly close to extinction. In this study, we reported first protocol of somatic embr...

Chrysanthemum morifolium propagation through stem cutting produces weak plants that show delayed anthesis above 20 °C and a reduced flower diameter. Therefore, in this study chrysanthemum plantlets were produced somatic embryogenic from calli to exploit the somaclonal variation for their improvement. Various explants variety Dante yellow cultured on Linsmaier Skoog (LS) medium with various conc...