The response of M. lyodeiktiwu to biotin or other nutrients was determined by turbidimetric measurement of growth. Five ml of test medium contained in aluminum-capped test tubes were used in each assay. Additions to 4 ml of basal medium varied the composition of the nutrient material. The basal medium (no. 1) (table 1) was stored under toluene in the refrigerator and aliquots withdrawn aseptica...

Recently, we reported that two homologous yeast proteins, Rai1 and Dxo1, function in a quality control mechanism to clear cells of incompletely 5' end-capped messenger RNAs (mRNAs). Here, we report that their mammalian homolog, Dom3Z (referred to as DXO), possesses pyrophosphohydrolase, decapping, and 5'-to-3' exoribonuclease activities. Surprisingly, we found that DXO preferentially degrades d...

Eukaryotic mRNAs generally possess a 5' end N7 methyl guanosine (m7G) cap that promotes their translation and stability. However, mammalian mRNAs can also carry a 5' end nicotinamide adenine dinucleotide (NAD+) cap that, in contrast to the m7G cap, does not support translation but instead promotes mRNA decay. The mammalian and fungal noncanonical DXO/Rai1 decapping enzymes efficiently remove NA...

Approximately 700 nucleotide sequences surrounding the transcription initiation site were determined with a cloned rDNA fragment of Tetrahymena pyTiformis and the transcription initiation site was localized on these sequences using purified 35S pre-rRNA. A considerable portion of the 35S prerRNA was found to be capped in vitro. The P-labeled, capped 35S pre-rRNA, on nuclease PI protection mappi...

Thermal buckling behavior of truncated conical liner reinforced by laminated composite is investigated in the presence of a general initial imperfection. For this purpose, the method of virtual work and first-order strain-deformation shell theory are employed to extract equilibrium equations. To this end, a finite element code is developed using the 3D 8-node shell element with six degrees of f...

Soft amphiphilic nanotubes are capped with vesicles comprised of either overall neutral, zwitterionic phospholipids, or those that carry a net charge. The phase transition temperature of the zwitterionic phospholipids plays a crucial role in the phase separation that leads to the end-capped nanotubes. The cationic vesicle caps can be loaded into the nanotubes via osmosis whereas the anionic ves...

This paper studied Buckling analysis of porous truncated conical shell subjected to axial load. It is considered that a fluid undrained between porous material and the Porous material properties vary across the thickness of shell with a specific function also assumed that the edge of the shell is simply supported. The governing equations are based on the Sanders kinematics equations and the fir...