an amplification polymerase chain reaction (pcr) test for the direct detection of chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. during 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specimens from ...

The commercial COBAS Amplicor CT/NG test (Roche Diagnostic Systems, Meylan, France) is a sensitive and specific method for detection of Chlamydia trachomatis infections. This test currently consists of using a nucleic acid amplification method to detect C. trachomatis in first-void urine specimens and in endocervical swabs collected in 2-sucrose-phosphate (2SP) transport medium. We conducted a ...

The fully automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for the ability to detect Chlamydia trachomatis infections. Test performance compared to that of culture was evaluated for 2,236 matched endocervical swab and urine specimens obtained from women and for 1,940 matched urethral swab and urine specimens obtained from men. Culture-n...

The incidence of candida infections is on the increase globally, with laboratories not performing speciation and susceptibility testing routinely. This study aims to speciate determine profile yeast isolates recovered from urogenital specimens. A total 115 female participants signs symptoms tracts infection attending various clinics in University Benin Teaching Hospital City, Nigeria were recru...

Objectives-To evaluate the role of detecting asymptomatic bacteriuria and endocervical infections in the black prenatal patients attending King Edward VIII Hospital (KEH), Durban, with the view of justifying a screening programme. Screening for syphilis and human immunodeficiency virus (HIV) infection were also evaluated. Subjects-181 asymptomatic black prenatal patients attending the antenatal...

Background: The majority of Chlamydia trachomatis infections in women are asymptomatic, but they may give rise to tubal infertility. Screening programmes aim at reducing morbidity in individual by early detect and treatment, and at decreasing the overall prevalence of infection in the population. Materials and Methods: A total of 135 women presenting with a history of infertility(n=113(83.7%) p...

A coamplification PCR test for the direct detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urethral and endocervical swabs and urine samples from men and women was compared to standard culture techniques. Processed specimens were amplified in single reaction tubes containing primers for both organisms, and PCR products were detected by a colorimetric microwell plate hybridization...

Forty five (37%) of 121 female contacts of men with non-gonococcal urethritis or gonorrhoea were chlamydia positive, as judged by isolation or by detecting elementary bodies in smears with a fluorescein labelled chlamydial monoclonal antibody. Only six (13%) of these, however, had Papanicolaou stained smears in which there were inclusion like changes suggestive of chlamydial infection. Furtherm...

Introduction. Preterm premature rupture of membranes (PPROM) usually has a multifactorial etiology that is often unknown, although the most frequently reported cause is infection by group B Streptococcus. Therefore, the etiology of PPROM, although probably infectious, remains unknown in most cases. This case describes a PPROM caused by infection from oropharyngeal microbiota. Case presentation....

We read with interest the article by Safaeian and colleagues (1). Using data from the Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesions Triage Study (ALTS), they show that diagnosis of cervical intraepithelial neoplasia grade 3 or worse (CIN3+) due to human papillomavirus (HPV)-18 is delayed compared with HPV16 lesions. It has been suggested that th...