as igm and iga-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of igm and iga enriched immunoglobulins. fraction iii which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using sephadex deae a-50 and 0.1 m tris-d.35m naci buff...

in this study the optimal amount of enriching artemia franciscana with a synbiotic combination of pediococcus acidilactici and fructooligosaccharide (fos( was investigated. the experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics p. acidilactici (1×109, 1×108 and 1× 107 cfu per l) and multi-level enriched prebiotic fos (1, 2 and 5) g per l of solu...

background: platelet preparations have been used for a wide variety of clinical applications such as hemorrhage, tissue engineering and cellular therapy. platelet concentrates can be prepared by the apheresis method or from the whole blood using the buffy-coat or platelet-rich plasma methods. the purpose of this study was to compare four variations of platelet-rich plasma method based on double...

the present study investigates the possibility of enriching adult artemia franciscana with singular or combined administration of pediococcus acidilactici and fructooligosaccharide (fos). the experiment was conducted in a completely randomized design with four treatments, including synbiotic, p. acidilactici +fos (t1), probiotic, p. acidilactici (t2), prebiotic, fos (t3) and control (t4). to ev...

While apoliprotein E (ApoE) epsilon 4 allele is now a well established risk factor for familial and sporadic senile Alzheimer's disease (AD), its role in the development of the rarer presenile or early onset type is controversial. Early studies showed no association; later ones found enrichment for the epsilon 4 allele in familial or sporadic types or both. We have ApoE genotyped a series of Sc...

In this study, we present the first experimental results for stable barium (Ba) isotope ((137)Ba/(134)Ba) fractionation during low-temperature formation of the anhydrous double carbonate BaMn[CO(3)](2). This investigation is part of an ongoing work on Ba fractionation in the natural barium cycle. Precipitation at a temperature of 21±1°C leads to an enrichment of the lighter Ba isotope described...

Supplemental Methods 15 Sperm sample collection and library preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Hierarchical clustering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Orthologous promoter HMR sizes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Ultra-conserved HMRs . . . . . . . . . . . . . . . . . . . ...

Nutrient availability influences an organism's life history with profound effects on metabolism and lifespan. The association between a healthy lifespan and metabolism is incompletely understood, but a central factor is glucose metabolism. Although glucose is an important cellular energy source, glucose restriction is associated with extended lifespan in simple animals and a reduced incidence o...

Genetic analysis of von Willebrand disease by von Willebrand factor gene sequencing has not yet become routine practice. Nevertheless, the prospects for molecular diagnosis have changed dramatically in recent years with the unveiling of next-generation sequencing platforms. With the goal of applying this technology to von Willebrand disease, we designed a strategy for von Willebrand factor gene...