The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on the electrode's surface through electropolymerization with m-phenylenediamine. The enzyme biosensor was optimized and tested in model glucose samples. The bi...

We have modified an ultramicro method for determining glucose with glucose oxidase-peroxidase-odianisidine. Capillary blood is diluted in isotonic NaF (17.6-17.8 g/liter), the cells are removed, and glucose is determined after a 20-mm incubation with enzyme-containing reagent. Increasing the enzyme concentration fourfold nullifies interference from ascorbic acid and hemolysis, while greatly dec...

Rapid measurement of glucose, glutamate, and lactate is important in understanding the dynamics of the energy balance of the brain. Glutamate is also the main excitatory neurotransmitter. A general immobilized enzyme-based flow injection assay system is described which uses oxidase and peroxidase enzymes to convert the analyte into an oxidized ferrocene species which is detected electrochemical...

The electrostatic assembly of nanocomposite thin films consisting of alternating layers of an organometallic redox polymer (RP) and oxidoreductase enzymes, glucose oxidase (GOX), lactate oxidase (LOX) and pyruvate oxidase (PYX), was investigated. Multilayer nanostructures were fabricated on gold surfaces by the deposition of an anionic self-assembled monolayer of 11-mercaptoundecanoic acid, fol...

A D-erythorbic acid-forming soluble flavoprotein, gluconolactone oxidase (GLO), was purified from Penicillium cyaneo-fulvum strain ATCC 10431 and partially sequenced. Peptide sequences were used to isolate a cDNA clone encoding the enzyme. The cloned gene exhibits high levels of similarity with the genes encoding other known eukaryotic lactone oxidases and also with the genes encoding some puta...

Compartmentalization is a ubiquitous feature of life, with membrane interface that regulates molecular transport and exhibits bioactivities. An artificial enzyme-integrated cascade system can mimic such interactive properties across different length scales for in vitro application. Here, it shown reticulated nanoporous framework nano-caged enzymes presents the first modular macroscale platform ...

The scanning electrochemical microscope (SECM) is used to image the activity of enzymes immobilized on the surfaces of disk-shaped carbon-fiber electrodes. SECM was used to map the concentration of enzymatically produced hydroquinone or hydrogen peroxide at the surface of a 33-microm diameter disk-shaped carbon-fiber electrode modified by an immobilized glucose-oxidase layer. Sub-monolayer cove...

Corresponding Author: Christina Schmid, Ph.D., Institut für Diabetes-Technologie Forschungsund Entwicklungsgesellschaft mbH an der Universität Ulm, Helmholtzstrasse 20, 89081 Ulm, Germany; email address [email protected] Many people with diabetes routinely measure their blood glucose (BG) on capillary blood samples from the fingertip. Beside other interfering factors, the blood sample...