Primary cultures of bovine adrenal chromaffin cells provide large quantities of a homogeneous population of target cells for nerve growth factor (NGF) and, thus, are a suitable system for studying the molecular mechanism of action of NGF. In this study, we have shown that NGF mediates the specific induction of the key enzymes in catecholamine biosynthesis, tyrosine hydroxylase (TH), dopamine-be...

A quantitative understanding of microbial growth on mixtures of substrates is of considerable biological and practical interest. Microbes growing in mixed-substrate environments display a rich spectrum of dynamics. The goal of this work is to show that it is possible to capture these dynamics by a judicious choice of only a few physiological variables. The central idea of the mathematical model...

In this study, Rhizomucor miehei ?-1,3-1,4-glucanase gene was expressed under the regulation of AOX1 promoter in Pichia pastoris expression system, and clone providing highest production level determined. The codon-optimized ligated into vector pPICZ?A transferred competent P. KM71H cells. Thirty transformants were selected from plates containing different concentrations zeocin cultured test tu...

One of the major biochemical effects of PCBs is the induction of microsomal enzymes in the liver. Risebrough et al. (1) suggested that PCBs have the capability to induce the activities of microsomal enzymes. Subsequently, Street et al. (2), demonstrated the induction of liver enzymes in rats by PCBs. Since then, a great number of articles have been published on this subject. The enzyme systems ...

Due to the importance of in vitro cytochrome P450 (P450) induction assay to assess the possible drug-drug interaction events, the recent US Food and Drug Administration draft guidance and European Medicines Agency guideline recommend to assess P450 induction using fresh or cryopreserved hepatocytes at mRNA level and/or enzyme activity level. Although cryopreserved hepatocytes are commercially a...

Due to the importance of in vitro cytochrome P450 (P450) induction assay to assess the possible drug-drug interaction events, the recent US Food and Drug Administration draft guidance and European Medicines Agency guideline recommend to assess P450 induction using fresh or cryopreserved hepatocytes at mRNA level and/or enzyme activity level. Although cryopreserved hepatocytes are commercially a...

Due to the importance of in vitro cytochrome P450 (P450) induction assay to assess the possible drug-drug interaction events, the recent US Food and Drug Administration draft guidance and European Medicines Agency guideline recommend to assess P450 induction using fresh or cryopreserved hepatocytes at mRNA level and/or enzyme activity level. Although cryopreserved hepatocytes are commercially a...

Due to the importance of in vitro cytochrome P450 (P450) induction assay to assess the possible drug-drug interaction events, the recent US Food and Drug Administration draft guidance and European Medicines Agency guideline recommend to assess P450 induction using fresh or cryopreserved hepatocytes at mRNA level and/or enzyme activity level. Although cryopreserved hepatocytes are commercially a...

Due to the importance of in vitro cytochrome P450 (P450) induction assay to assess the possible drug-drug interaction events, the recent US Food and Drug Administration draft guidance and European Medicines Agency guideline recommend to assess P450 induction using fresh or cryopreserved hepatocytes at mRNA level and/or enzyme activity level. Although cryopreserved hepatocytes are commercially a...