The effect of Annona squamosa, Lawsonia inermis and their combination with other herbal molluscicides were studied on different enzyme activity in the nervous tissue of Lymnaea acuminata. Twenty-Four hour in vivo exposure to 40% and 80% of 24 h LC50 of plant derived molluscicides and their combination with other molluscicides such as Cedrus deodara, Azadirachta indica oil, Allium sativum, Polia...

Monoamine oxidases (MAOs) and muscarinic acetylcholine receptors (mAChRs) are considered important therapeutic targets for Parkinson’s disease (PD). Lipophilic tanshinones major phytoconstituents in the dried roots of Salvia miltiorrhiza that have demonstrated neuroprotective effects against dopaminergic neurotoxins inhibition MAO-A. Since MAO-B is an effective strategy PD, we tested inhibitory...

Background: Phytocystatins are plants cysteine protease inhibitors (CPIs) that known for their numerous uses in medicine and biotechnology. Methods: Different extraction media which include, Sodium Hydroxide, Hydrochloric acid, Chloride, phosphate buffer distilled Water were used to evaluate flower, leave, root, latex, stem bark of Moringa oleifera inhibitory activity against (Papain enzyme). T...

The enzyme uridine diphosphate glucose pyrophosphorylase has been prepared at high purity (specific activity 127) from the human erythrocyte. Comparison of its specific properties with enzyme isolated from liver indicates many similarities including size. Examination of the reversible reaction catalyzed by the erythrocyte enzyme from both forward and reverse directions revealed a highly selecti...

The pivotal role played by ω-transaminases (ω-TAs) in the synthesis of chiral amines used as building blocks for drugs and pharmaceuticals is widely recognized. However, bulky are challenging to produce. Herein, a ω-TA (TR8) from marine bacterium was synthesize fluorine amine ketone. An analysis reaction conditions process development showed that isopropylamine concentrations above 75 mM had an...

Purpose: We conducted a study that examined the physicochemical properties of Eriobotrya japonica leaf enzyme-assisted extract (EJLE) from Jeju Island.Methods: EJLE was prepared using pepsin enzyme. The antioxidant and antiaging effects were investigated to determine its utility as functional material.Results: In analysis total amino acids, 16 kinds components isolated EJLE. DPPH ABTS radical s...

Metabolites can inhibit the enzymes that generate them. To explore the general nature of metabolic self-inhibition, we surveyed enzymological data accrued from a century of experimentation and generated a genome-scale enzyme-inhibition network. Enzyme inhibition is often driven by essential metabolites, affects the majority of biochemical processes, and is executed by a structured network whose...

By use of different inhibitors as well as atomic absorption spectrophotometry it has been shown that the haemolymph-acetylcholinesterase (E. c. 3.1.1.7) of the sea mussel Mytilus edulis is a metalloprotein containing 2,95 Fe2+-ions per subunit. All inhibitors used (1,10-phenanthroline, salicylic aldehyde, 2,2'-dipyridyl, 8-hydroxyquinoline) showed a non-competitive inhibition, which was not pH-...

Choline-phosphate cytidylyltransferase (EC 2.7.7.15) activity from 25- and 29-day-foetal rabbit lungs was inhibited in both the cytosolic and the microsomal fractions by preincubation with MgATP. The inhibition of the cytosolic enzyme was greater when measured with added phosphatidylglycerol (PG) than without (78-89% versus 50-55%), whereas the inhibition of the microsomal enzyme did not exhibi...