Bovine monoamine oxidase (MAO) B has been synthesized in vitro using a reticulocyte lysate translation system directed by bovine liver poly(A)+ RNA. The newly synthesized enzyme apparently lacks a cleavable N-terminal extension, but MAO B is readily incorporated into mitochondria or isolated mitochondrial outer membranes prepared from rat liver. ATP is not required for the binding of the newly ...

A nanosensory membrane device was constructed for detecting liposome fusion through changes in an enzymatic activity. Inspired by a biological signal transduction system, the device design involved functionalized liposomal membranes prepared by self-assembly of the following molecular components: a synthetic peptide lipid and a phospholipid as matrix membrane components, a Schiff's base of pyri...

Collagen IV comprises the predominant protein network of basement membranes, a specialized extracellular matrix, which underlie epithelia and endothelia. These networks assemble through oligomerization and covalent crosslinking to endow mechanical strength and shape cell behavior through interactions with cell-surface receptors. A recently discovered sulfilimine (S=N) bond between a methionine ...

Abstract The regulation of the activity and selectivity phospholipase A2 (PLA2), which is capable cleaving fatty acid in second position ( sn-2 ) phospholipid, carried out through membrane-binding catalytic sites enzyme. For hydrolytic activity, PLA2 must first bind to phospholipid membrane, binding efficiency depends on composition membrane. site formed by several tens amino acids its differs ...

The chlorophyll precursors protochlorophyllide and chlorophyllide were identified in purified envelope membranes from spinach (Spinacia oleracea) chloroplasts. This was shown after pigment separation by high performance liquid chromatography (HPLC) using specific fluorescence detection for these compounds. Protochlorophyllide and chlorophyllide concentrations in envelope membranes were in the r...

A strategy for the immobilization of enzyme 2-deoxy-d-ribose-5-phosphate aldolase (DERA) is presented via spray-coating on membrane support material to generate an enzymatically active which can be used in continuously run synthesis modules. functional, water-soluble copolymer containing addressable units covalently bind DERA mixed with enzyme, followed by mixture onto polyacrylonitrile/polyeth...

The principle endocannabinoids are 2-acylglycerol esters, such as 2-arachidonoylglycerol (2-AG), and N-acylethanolamines, anandamide (N-arachidonoylethanolamine, AEA). glycerol esters ethanolamides synthesised hydrolysed by parallel, independent pathways. Mechanisms for release re-uptake of unclear, although potent selective inhibitors facilitated diffusion across cell membranes have been devel...

The structural organization of crystalline, membrane-bound (Na+ + K+)-ATPase was studied by negative staining and thin sectioning. The enzyme molecules were induced to form crystalline arrays within fragments of membrane by incubation in defined ionic conditions. The enzyme remained fully active after crystallization. Negative staining and computer processing of images of the crystalline specim...

The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of at least 14 subunits (NQO1-14) and is located in the cytoplasmic membrane. In the present study, topological properties and stoichiometry of the 7 subunits (NQO1-6 and NQO9) of the P. denitrificans NDH-1 in the membranes were investigated using immunological techniques. Treatments with chaotr...