Enzyme and Cell Technology Group, Department of Biochemistry and Molecular Biology, 8 Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 9 P.O. Box 644, E-48080 Bilbao, Spain 10 Department of Environmental Biology, Biological Research Center, CSIC, C/. Ramiro de 11 Maeztu, 9, Madrid E-28040, Spain 12 Department of Biochemistry and Molecular Biology I, Faculty of Biol...

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...

An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...

deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. dna-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy. in this study universal primers common to almost all fungi were used to amplification of internal transcribe spacer 1 and 2 region. subsequent restriction enzym...

ceruloplasmin (cp) is a human plasma protein with multiple physiological functions including ferroxidase and oxidase activities. deficiency or inhibition of the enzyme may lead to some abnormalities, including iron deposition in several tissues and causes various pathological conditions in the body. direct interaction of cadmium (cd), a widespread, highly toxic environmental pollutant, with hum...

the present study was laid out in order toevaluate the effect of salinity on some biochemical characterize of grapevine c.vyaghouti. the experiment was done at agricultural and natural resources research center, ilam, iran at 2014. the experiment was a completely block design (crd) with four replications. saline factor was nacl salinity in seven levels (zero, 50, 100, 150, 200 and 250 mm). grap...

background: growth promoters are chemical and biologicalsubstances that are added to livestock food with the aimto improve the growth of chickens in fattening and the utilizationof food, and in this way achieve better production and financialresults. objectives:an experiment was conducted to evaluatethe effects of enzyme complex, probiotic, and their combinationon performance of broilers fed a ...

The porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2 were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as follows: hexadecyltrimethyl ammonium bro...

DNA sequencing is one of the most important platforms for the study of biological systems today. Sequence determination is most commonly performed using dideoxy chain termination technology. Recently, pyrosequencing has emerged as a new sequencing methodology. This technique is a widely applicable, alternative technology for the detailed characterization of nucleic acids. Pyrosequencing has the...

We describe a new discovery technology that uses mRNA-display to rapidly synthesize and screen macrocyclic peptide libraries to explore a valuable region of chemical space typified by natural products. This technology allows high-affinity peptidic macrocycles containing modified backbones and unnatural side chains to be readily selected based on target binding. Success stories covering the firs...