The kinetics of drug-receptor interactions can profoundly influence in vivo and in vitro pharmacology. In vitro, the potencies of slowly associating agonists may be underestimated in assays capturing transient signaling events. When divergent receptor-mediated signaling pathways are evaluated using combinations of equilibrium and transient assays, potency differences driven by kinetics may be e...

The term Ergot is referred to the sclerotium of ascomycetes - a protective kernel produced during resting stage of some fungi - which replaces seeds of susceptible cereals and plants intended for human and animal diet. It contains various composition of tryptophan-derived toxins defined ergot alkaloids. Since sclerotia can be harvested and milled together with cereals, they represent a source o...

A liquid chromatography/ion trap mass spectrometry-based method to determine six ergot alkaloids and their isomers is presented. The samples were cleaned on neutral alumina-based solid-phase extraction cartridges. The following method parameters were obtained (depending on the analyte and spiking level): method recovery from 63.0 to 104.6%, relative standard deviation below 18%, linear range fr...

Five tall fescue [Lolium arundinaceum (Schreb.)] pastures [wild-type 'Kentucky-31' with 78.0% of plants infected with ergot alkaloid-producing endophyte Neotyphodium coenophialum (KY31 E+), 'Jesup' AR542 endophyte-infected contaminated with 30.3% tall fescue containing ergot alkaloid producing-endophyte (Jesup AR542 E+); 'GA-186' AR584 endophyte-infected contaminated with 11.8% tall fescue cont...

Ergot alkaloids are hypothesized to cause vasoconstriction in the midgut, and prior exposure may affect the vasoactivity of these compounds. The objectives of this study were to profile vasoactivity of ergot alkaloids in bovine mesenteric artery (MA) and vein (MV) and determine if previous exposure to endophyte-infected tall fescue seed affected vasoactivity of ergocryptine (ERP), ergotamine (E...

Ergot alkaloids (EAs) are secondary metabolites produced by ergot fungi (e.g., Claviceps purpurea), which parasites of Gramineae grasses. EAs and their analogs used to treat migraine, postpartum uterine bleeding, Parkinson's syndrome. Recent studies have reported additional new bioactive activities analogs, making them essential compounds for drug development, repositioning, clinical applicatio...

Abstract The objective of this study was to evaluate the effect feeding pelleted or mash ergot contaminated grain continuously intermittently on performance and blood parameters backgrounding steers. Sixty black angus steers (300 ± 29.4 kg) were used in a complete randomized (60% barley silage:40% concentrate DM basis) 84 d study. Steers randomly assigned 4 different treatments housed individua...

Ergot alkaloids (EAs) are mycotoxins produced by several species of fungi the genus Claviceps , among which purpurea is most widespread in Europe. This has been found many economically important cereal grains, such as rye, wheat, triticale, barley, millet and oats. The distribution EAs contamination a sporadic incidence with factors involved its occurrence, greatly varying between fungal strain...

On referring to seventeen obstinate cases of menorrhagia, which. I treated during the last two years with ergot of rye and Indian hemp, I am in a position to corroborate the statement made in your last number by Mr. Bensley, that tincture of the Indian hemp is a very valuable medicine " for the treatment of menorrhagia and uterine haemorrhages generally," the latter of course not depending on a...