The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an vitro open environment without complete living cell. It has been widely used basic applied research fields because its advanced engineering features flexibility controllability. Compared to typical crude extract-based CFPS due defined customizable...

Using nylon bag techniques, Cornell net carbohydrates and protein systems (CNCPS), scanning electron microscopy, the authors examined digestibility structure of Vicia ervilia (ervil, bitter vetch) after steam flaking, roasting, microwave processing. During in situ technique, samples were incubated at 0, 2, 4, 6, 8, 12, 16, 24, 36, 48 h. For description ruminal DM (dry matter) CP (crude protein)...

A number of analytical methods for constituents commonly measured in distillers dried grains (DDG) are practiced in laboratories serving the agricultural sector. A large interlaboratory variability among results has been observed in the industry. Methods for moisture, crude fat, and crude fiber are empirical, thus part of this variability can be attributed to the use of different methods of ana...

The changes in crude protein, free amino acids, amino acid composition, protein solubility, protein fractionation and protein digestibility after germination of sorghum were investigated. Sorghum varieties (Dorado, Shandaweel-6, Giza-15) were soaked for 20 h followed by germination for 72 h; the results revealed that crude protein and free amino acids in raw sorghum varieties ranged from 10.62 ...

This paper is a report on the nutritional composition (dry matter, total protein, fat, crude fibre, ash, β-glucan content, and selected mineral compounds), antioxidant (antioxidant activity with DPPH method anthocyanin content), physical properties, sensory profiles of prepared bread enriched black oat flours (variety Norik Hucul) in amounts 3, 6, 9%. In breads (especially 9% addition), there w...

In this study, the effects of different cellulosic wastes on nutritional values Pleurotus ostreatus (Jacq.) P. Kumm. were investigated. Dry matter, moisture, protein, fat and ash contents analyzed according to AOAC methods. Crude protein determined by Kjeldahl method Soxhlet extraction, using ether as a solvent, respectively. Chemical composition such dry crude organic matter 93.5-93.7%, 6.3-6....

The effect of grape pomace (Vitis vinifera) and in oak leaf (Quercus libani) tannins by three runs of in vitro gas production on the organic matter digestibility (OMD) and in situ dry matter (DM) and crude protein (CP) disappearance were studied in four sheeps. Polyethylene glycol (PEG) was used to deactivate the tannins. In vitro gas production was recorded at 2, 4, 6, 8, 12, 48, 72, 96 and 12...

This study was conducted to determine in vitro crude protein degradation (IVDP) parameters and effective crude protein degradability (EPD) of various feeds using the modified in vitro gas production (GP) technique. Feed samples were alfalfa hay, soybean meal, soybean, rapeseed meal, sunflower meal and fish meal. Rumen fluid was collected before the morning feeding from four rumen fistulated lam...

thirty multiparous holstein dairy cows with average body weights of 687±15 kg and body condition scores of 3.25±0.15 were allocated to 1 out of each 3 groups, immediately after parturition (dim=11) and in a completely randomized design. the first group was fed diet low in rumen undegradable protein (rup; 5.1% of dry matter), the second group fed with medium rup (7.2% of dry matter) while the th...