This study applied response surface methodology and synthetic evaluation method to optimize the alkaloid content of buckwheat sprouts. Solid-liquid (1:10-1:30), ethanol concentration (60%-80%), extraction temperature (20°C-60°C) and extraction time (20-40 min) were considered as treatment factors in the optimization. All treatment variables shown significant effects on the alkaloid content of b...

The separation of liver ribonucleic acid and deoxyribonucleic acid from the associated protein material without hydrolysis can be accomplished by a salt-extraction procedure described by Barnum & Huseby (1950). This method, based on ribose recovery (Lowe & Rand, 1956; C. U. Lowe & P. R. Venkataraman, in preparation), yields 80% of the total ribonucleic acid of normal liver homogenate or cell fr...

Fermentative bioethanol production from pyrolytic sugars was improved via aromatics removal by liquid-liquid extraction. As solvents, the ionic liquid (IL) trihexyltetradecylphosphonium dicyanamide (P666,14[N(CN)2]) and ethyl acetate (EA) were compared. Two pyrolytic sugar solutions were created from acid-leached and untreated pinewood, with levoglucosan contents (most abundant sugar) of 29.0% ...

BACKGROUND Biomass refining into multiple products has gained considerable momentum due to its potential benefits for economic and environmental sustainability. However, the recalcitrance of biomass is a major challenge in bio-based product production. Multilevel composition fractionation processes should be beneficial in overcoming biomass recalcitrance and achieving effective conversion of mu...

In the present study, various mixtures of aqueous ethanol and acetone solutions were utilized to extract anthocyanins from eggplant peel by a discontinuous process to obtain a natural red-purple colorant. First, the most appropriate combination for extraction solvent was selected as ethanol 70 wt in water acidified with acetic acid 3 wt, which was determined by analysis of extraction yields and...

Auto-catalyzed hydrothermal pretreatment (A-HTP) and sulfuric-acid-catalyzed (SA-HTP) were applied to quinoa stalks in order reduce their recalcitrance towards enzymatic saccharification. Prior pretreatment, extracted with either water or a 50:50 (v/v) ethanol–water mixture for removing saponins. Extraction aqueous ethanol, respectively, led removal of 52 75% (w/w) the saponins contained raw ma...

Gloriosa superba is a medicinal plant used in traditional medicine for the treatment of various diseases. The tuber of Gloriosa superba is a rich source of colchicine which is used for the treatment of gout, cirrhosis and also used in plant breeding studies to produce polyploidy. Optimization of various extraction parameters using response surface methodology (RSM) was performed to assess maxim...

The effects of different solvents, temperature conditions, solvent-solid ratios and particle sizes on solid-solvent extraction of the total phenolics, proanthocyanidins and flavonoids herein also referred to as antioxidant from pomegranate marc peel (PMP) was studied. Water, methanol, ethanol, acetone, and ethyl acetate extraction efficiencies at extraction times of 0.17 to 10 min, extraction t...

obtained without a phenol/chloroform extraction; however, fewer errors, greater signal strength and longer reads are obtained from the inclusion of a phenol/chloroform extraction before the butanol precipitation (Figure 1, A and C). In conclusion, this paper presents a simple dye terminator sequencing product purification protocol. This protocol is standardly used in our laboratory and provides...