The [3 + 2] cycloaddition reaction between carbonyl ylides generated from epoxides and ketones (ethyl pyruvate, ethyl phenylglyoxylate, isatin, N-methylisatin and 5-chloroisatin) to give substituted dioxolanes and spirocyclic dioxolane indolinones was investigated. The effect of microwave irradiation on the outcome of the reaction was studied. The thermal reaction between 2,2-dicyano-3-phenylox...

pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and adp to yield atp and pyruvate. geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. given that limited proteolysis experiments can be successfully used to probe conformational features of p...

2,3-dihydroxy-2-methylbutyric acid, also known as 2,3-dimethylglyceric constitutes the acyl and/or alcoholic moiety of many bioactive natural esters. Herein, we describe a chemoenzymatic methodology which gives access to all four possible stereoisomers acid ethyl ester. The racemic ?-acetolactate, produced by N-heterocycle carbene (NHC)-catalyzed coupling pyruvate and methylacetoin was employed...