BACKGROUND Enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli epithelial cell adhesion is characterised by intimate attachment, and attaching and effacing (A/E) lesion formation. This event is mediated in part by intimin binding to another bacterial protein, Tir (translocated intimin receptor), which is exported by the bacteria and integrated into the host cell plasma membra...

Gel-filtration chromatography of culture medium from rabbit bone explants separates three latent metalloproteinases with activities against collagen, proteoglycan and gelatin respectively. The fractions degrading proteoglycan also degrade laminin, fibronectin and the polymeric products of pepsin-solubilized type IV collagen and can also solubilize insoluble type IV collagen. The fractions degra...

Methods Explants of normal porcine vaginal mucosa (full-thickness) were infected with biofilm-producing methicillinresistant S. aureus (MRSA) (3.2x10^5 CFU) for 2h – 48h. Following infection (2h), explants were treated with the active dressing (TegadermTM CHG) for 22-46h or left untreated (controls). Formation of MRSA biofilm was evaluated by scanning electron microscopy. Also, bacteria were en...

Cereus peruvianus (Cactaceae) is an important medical plant. The study was carried out on callus induction of Cereus jamacaru f. monstrosus and Cereus hildmannianus fma monstrosa (Cereus peruvianus). Apical and lateral explants were cultured on Murashige and Skoog media with factorial combinations of the auxins indole-3acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and N-(2-furanyl-...

Conservation and propagation of species using biotechnologic tools-such as plant tissue culture-are relevant when natural propagation is hampered for various reasons. In vitro techniques allow mass multiplication and propagation under pathogen-free conditions but also override dependence on season for availability of plant material. Moreover, in vitro genetic manipulation of a species, invariab...

The purpose of the research study is to select the best rice explant and variety for Agrobacterium mediated genetic transformation studies. In vitro callus induction and regeneration frequency of two explants i.e. immature embryo and mature grains of 9 rice varieties (Oryza sativa L.) was investigated by culturing explants source on N6 media supplemented with 2, 4-D at 2mg/l for callus inductio...

Ethylene stimulated RNA and protein synthesis in bean (Phaseolus vulgaris L. var. Red Kidney) abscission zone explants prior to abscission. The effect of ethylene on RNA synthesis and abscission was blocked by actinomycin D. Carbon dioxide, which inhibits the effect of ethylene on abscission, also inhibited the influence of ethylene on protein synthesis. An aging period appears to be essential ...

In traditional in vitro culture, explants grow enclosed in a non-ventilated vessel at high relative humidity with phytohormones continuously present and sucrose as the main energy source. Under such conditions explant growth is far from normal. In this paper, explants of Actinidia deliciosa were cultured in MS medium supplemented with sucrose, benzyladenine and gibberellic acid under autotrophi...

PURPOSE To explore the effect of docosahexaenoic acid (DHA) on gene expression during human fetal retinal maturation. METHODS Human fetal retinal explants were cultured in serum-free Waymouth's medium supplemented with DHA or oleic acid (OA), using bovine serum albumin (BSA) as the vehicle. After 14 days in culture, fatty acid composition was assessed, and the abundance of 2400 cDNAs was exam...

OBJECTIVE To determine the influence of IL-1 beta on the presence and the distribution of tenascin in matrix of human normal and osteoarthritic cartilage explants. METHODS Cartilage was grown in organotypic culture with or without IL-1 beta (10 ng ml1). Tenascin antigen was detected on cryopreserved cartilage sections by immunohistochemical techniques with a monoclonal antibody directed again...