The liver fluke, Fasciola hepatica, is considered as the most common cause of fasciolosis in both domestic livestock and human. This study was carried out to detect the prevalence of the larval stages of F. hepatica in the snails Galba truncatula and Lymnaea stagnalis in West Azarbaijan, Iran. Snail collection was performed through searching 28 freshwater habitats from May to December 2010. Fol...

Cathepsin L1, a cysteine protease secreted by the gastrodermis of juvenile and adult Fasciola gigantica, was expressed in Escherichia coli as a calmodulin binding peptide fusion protein with a molecular mass of approximately 35 kD. The recombinant cathepsin L1 (rCTL1) was tested for its antigenic potential in a cystatin capture enzyme-linked immunosorbent assay (ELISA) to diagnose human fasciol...

Lymnaea (Radix) auricularia rubiginosa (Michelin, 1831) was surveyed in 54 reservoirs of 18 districts in Amphoe Muang, Khon Kaen Province during February to May 1994. Lymnaeid snails were found in the water of 20 reservoirs, of which 16 reservoirs contained clear water and 4 turbid water. Two of the four turbid water reservoirs received drainage water from Khon Kaen Town. Two thousand four hund...

A study was conducted to determine the kinetics of Fasciola gigantica infection induced antibody response against oxyclozanidelevamisole in naturally infected bovine of South Gujarat, India. Faecal analysis on before, 1st, 4th, 8th, 11th and 37th days post treatment (DPT) recorded 67.14, 34.29, 5.71, 0 and 0% parasites infected bovines. Either parasite(s) positive indigenous cattle or buffalo b...

A retrospective study covering a period of ten years (2001-2010) was conducted using postmortem meat inspection records of the Department of Veterinary Services in Gaborone to determine the prevalence of bovine fasciolosis in Botswana. Meat inspection records of monthly and annual returns from the two main export abattoirs in the country were examined, as well as the data collected on the total...

Fasciolosis is a parasitic helminthes disease caused by Fasciola gigantica. The different larval stage (sporocyst, redia and cercaria) are found in intermediate host snail Lymnaea acuminata. In the present study it has been tried to kill the F. gigantica larvae in vitro/ in vivo condition by the use of ferulic acid there is no mortality of snails. Mortality of larvae in in vivo and in vitro con...

Buffaloes are the important multipurpose farm animals in the Indian sub-continent, contributing signifi cantly to meat and milk production. Tropical fasciolosis caused by Fasciola gigantica is regarded as one of the most important diseases of buffaloes in humid tropical regions of the world. Bio-chemical changes are regarded as important indicators for actual pathogenesis and clinical diagnosis...

BACKGROUND We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method. METHODS We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle...

Gastrointestinal parasites are among the major causes of severe losses to livestock industry worldwide, especially in small ruminants. This study was conducted to determine the prevalence of gastrointestinal parasites in goats kept by small holders in the urban and peri-urban areas of Mwanza City in Tanzania. Randomly, 36 farmers from urban areas, with a total of 280 goats and 22 farmers from p...

Nitric oxide (NO) and NO-related pathways have the potential to play critical roles in the helminthes life cycle. Nitric oxide and nitric oxide synthase(NOS) in the parasitic trematode Fasciola gigantica were investigated. Two forms of F. gigantica nitric oxide synthase were detected after chromatography on CM-cellulose. The NOS II had the highest specific activity. NOS II was purified to homog...