We studied the antimicrobial resistance and the molecular epidemiology of 99 enterococcal surveillance isolates from two hospitals of Brasilia, Brazil. Conventional biochemical tests were used to identify the enterococcal species and the disk diffusion method was used to determine their resistance profiles. Enterococcus faecalis (76%) and E. faecium (9%) were the most prevalent species. No ente...

PURPOSE This study was conducted in order to determine the occurrence of esp and biofilm formation among Enterococcus faecalis causing mastitis isolated from different bovine and environmental origins. MATERIALS AND METHODS A total of 41 E. faecalis isolates were obtained from clinical mastitis before antibiotic therapy, subclinical mastitis, dried manure bedding samples, and postpartum milk ...

Background and Aim: Because of complexity in root canals, irrigating solutions are needed in addition to mechanical instrumentation for thorough cleansing of the root canal system. This in-vitro study was designed to determine the inhibitory effect of arginine, protamine, and aqueous extracts of green tea and aloe vera against Enterococcus faecalis (E. faecalis), which causes endodontic failure...

Introduction: The purpose of this study was to compare the antibacterial effect of sodium hypochlorite gel and solution on Enterococcus faecalis (E. faecalis). The main purpose of a root canal treatment is to eliminate the bacteria and their products. Sodium hypochlorite solution has excellent antibacterial properties, but also some negative features. Materials and methods: Fifty six single...

The enterococcal surface protein, Esp, originally found in Enterococcus faecalis, has been associated with increased virulence, colonization, and biofilm formation (2, 3, 5, 6). Recently, a variant esp gene was found in Enterococcus faecium isolates from humans (L. Baldassarri, L. Burtuccini, M. G. Ammendolia, G. Gherardi, and R. Creti, Letter, Lancet 357:1802, 2001; R. J. Willems, W. Homan, J....

INTRODUCTION The aim of this in vitro study was to evaluate the antimicrobial activity of four intracanal medicaments on Enterococcus Faecalis (E. Faecalis). METHODS AND MATERIALS Fifty extracted single-rooted human teeth were prepared with standard method. After contaminating the canals with E. Faecalis, the samples were divided into one control and four experimental groups (n=10). The teeth...

The MICs and MBCs of CI-934, ciprofloxacin, difloxacin (A-56619), A-56620, norfloxacin, enoxacin, amifloxacin, and coumermycin were determined for 43 clinical isolates of Enterococcus faecalis known to be resistant to penicillin-aminoglycoside synergy. Results were compared with those obtained for 37 synergy-susceptible E. faecalis and 22 Enterococcus faecium strains. Although no substantial di...

We have cloned the DNA gyrase and topoisomerase IV genes of Enterococcus faecalis to examine the actions of quinolones against E. faecalis genetically and enzymatically. We first generated levofloxacin-resistant mutants of E. faecalis by stepwise selection with increasing drug concentrations and analyzed the quinolone resistance-determining regions of gyrA and parC from the resistant mutants. I...

OBJECTIVE To investigate the inhibition of Enterococcus faecalis by calcium peroxide (CaO₂). METHODS The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Enterococcus faecalis by CaO₂ and calcium hydroxide (Ca(OH)₂) were determined by direct exposure tests (n = 10). The inhibition zone of E. faecalis mycoderm treated with CaO₂ and Ca(OH)₂ paste (53% w/w) ...

Subgingival plaque samples and root canal samples were collected from 2,839 marginal periodontitis (MP) patients and 21 apical periodontitis (AP) patients. Enterococcus species were identified by a series of phenotypic and genotypic tests. Antimicrobial susceptibility assays were performed by an agar disk diffusion test. Multilocus sequence typing (MLST), eBURST, and minimum spanning tree were ...