(2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a potentially valuable liquid fuel and an excellent building block in asymmetric synthesis. In this study, cofactor engineering was applied to improve the efficiency of (2S,3S)-2,3-BD production and simplify the product purification. Two NADH regeneration enzymes, glucose dehydrogenase and formate dehydrogenase (FDH), were introduced into Escherichia c...

In this article we assess the effect of market imperfections and income inequality on rural production efficiency. The analysis is carried out using the notion of stochastic conditional efficiency computed in terms of free disposal hull (FDH) efficiency measurements. Free disposal hull and conditional FDH are output oriented with variable returns to scale. They are evaluated for rural productio...

Trade-off reviews the rate of marginal substitution of inputs and outputs onto the efficient frontier. On the other hand, marginal rates of substitution (MRS) are important quantities for analysts and managers. In this paper, by changing the method of Asmild (2004), an algorithm is found that it can be used to calculate the marginal rate of the free disposal hull (FDH) model.

We expressed the NH2-terminal domain of the multidomain, multifunctional enzyme, 10-formyltetrahydrofolate dehydrogenase (FDH), using a baculovirus expression system in insect cells. Expression of the 203-amino acid NH2-terminal domain (residues 1-203), which is 24-30% identical to a group of glycinamide ribonucleotide transformylases (EC 2.1.2.2), resulted in the appearance of insoluble recomb...