Detection of Toxoplasma gondii DNA in cat feces is considered indicative of the presence of T. gondii oocysts. This study aims to demonstrate that the high sensitivity of qPCR can lead to T. gondii DNA detection in cat feces in the absence of oocysts. A cat immune to toxoplasmosis was fed with a mouse experimentally infected with T. gondii. Detection of DNA of this parasite was performed by qPC...

The origin of Vaccinia virus (VACV) outbreaks in Brazil remains unknown, but since the isolation of VACV in Mus musculus mice during a zoonotic outbreak affecting cattle and milkers, peridomestic rodents have been suggested to be a link between cows and wild animals. Considering that experimentally infected mice eliminate viral particles in their feces, we investigated the presence of VACV in t...

An assay to identify Cryptosporidium DNA in bovine feces has been developed emphasizing standardization of sample preparation and simplification of the DNA recovery process for PCR amplification and DNA hybridization detection. The Cryptosporidium DNA recovery-PCR detection procedure (CR-PCR) can recover DNA suitable for PCR amplification without using or generating hazardous chemicals or waste...

The efficacy of orally given bicyclomycin on shigellosis was studied in rhesus monkeys infected with Shigella fIexneri 2a. All the animals developed lethal infection after rectal inoculation of the virulent strain 5503. Bicyclomycin and the kanamycin control were given orally at daily dosage of 40 mg;kg for a period of 5 days beginning 24 hours after the inoculation. Bicyclomycin compared favor...

Abstract Livestock waste such as feces, urine, and leftover feed left without further treatment can harm the environment in form of air, water, soil pollution. Therefore, efforts are needed to process livestock into useful products organic fertilizers. that has potential be a source fertilizer is etawa goat waste. Etawa feces processing technology bokashi made from fermented agricultural wastes...

Lev, Meir (Albert Einstein College of Medicine, New York, N.Y.), Raymond H. Alexander, and Stanley M. Levenson. Stability of the Lactobacillus population in feces and stomach contents of rats prevented from coprophagy. J. Bacteriol. 92: 13-16. 1966.-Lactobacilli were enumerated in the feces of rats prevented from coprophagy by tail-cupping. No differences were found when numbers of these organi...

Coprophagy occurs in a number of animal species, including nonhuman primates. During the 2003-2004 dry seasons at the Beza Mahafaly Special Reserve, Madagascar, we observed wild ring-tailed lemurs (Lemur catta) consuming dried fecal matter from three different species. Ring-tailed lemurs consumed human feces on 12 occasions, cattle feces twice, and feral dog feces once. Coprophagy in this popul...

A fecal analysis survey was undertaken to quantify animal inputs of pathogenic and indicator microorganisms in the temperate watersheds of Sydney, Australia. The feces from a range of domestic animals and wildlife were analyzed for the indicator bacteria fecal coliforms and Clostridium perfringens spores, the pathogenic protozoa Cryptosporidium and Giardia, and the enteric viruses adenovirus, e...

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10(4) 50% tissue cultur...

sodium isotopes were measured i n the pref l ight and pos t f l ight urine and feces, and those feces specimens collected during the manned Apollo 3 Trl missions, by analysis of the urine s a l t s and t h e raw feces i n large crysta3 multidimensional gamma-ray spectrometers. The 7 ~ e was chemically a 3-@I separated, and i t s concentration measured i n an a l l ~a1(1~1), anticoincidence Z sh...