نتایج جستجو برای: flp nested pcr rt pcr
تعداد نتایج: 223681 فیلتر نتایج به سال:
the invention of polymerase chain reaction (pcr) has been a milestone in the history of biological and medical sciences. the applications of pcr have not only completely revolutionised the research in the field of molecular genetics as well as animal and plant biotechnology, but the technique has also proved its relevance and ingenious utility in other fields of forensic sciences, molecular sys...
BACKGROUND Due to an increasing number of norovirus infections in the last years rapid, specific, and sensitive diagnostic tools are needed. Reverse transcriptase-polymerase chain reactions (RT-PCR) have become the methods of choice. To minimize the working time and the risk of carryover contamination during the multi-step procedure of PCR the multiplex real-time RT-PCR for the simultaneous det...
چکیده اسفناج (spinacia oleracea l.) یکی از مهمترین سبزیهای برگی است که به صورت تازه و یا فرآوری شده مصرف میشود و به دلیل داشتن خواص دارویی و مواد غذایی فراوان به صورت گسترده در دنیا کشت و مصرف میشود. گیاه اسفناج همواره تحت تاثیر بیمارگرهای مختلف قارچی، باکتریایی و ویروسی قرار میگیرد. در نمونهبرداریهای صورت گرفته از مزارع منطقه داوران شهرستان رفسنجان در سال1390، مجموعاً 177 نمونه از گیاها...
PURPOSE We present the largest study of both peripheral blood and lymph node samples examining the utility of reverse transcription-polymerase chain reaction (RT-PCR) for established molecular markers as a diagnostic tool in the molecular staging of prostate cancer patients undergoing radical prostatectomy. EXPERIMENTAL DESIGN Peripheral blood from 358 patients was obtained before radical pro...
Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum in brook trout tissue with gross clinical signs of disease by nested RT–PCR. Determination of par...
No abstract this article: A Nested RT-PCR Method for Confirming the Absence of all Three Types of Polioviruses in Primates and Human Cells Used in Vaccine Production
We have previously demonstrated that cytochrome P450 aromatase (P450arom) protein, an estrogen-synthesizing enzyme, is present and active in germ cells of the adult mouse testis. To establish that P450arom mRNA is expressed in germ cells of other species, we examined expression of P450arom in adult rat germ cells by employing reverse transcription-polymerase chain reaction (RT-PCR). Total RNA w...
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