A combined approach based on isothermal titration calorimetry (ITC), fluorescence resonance energy transfer (FRET) experiments, circular dichroism spectroscopy (CD), atomic force microscopy (AFM) dynamic force spectroscopy (DFS), and surface plasmon resonance (SPR) was applied to elucidate the mechanism of protein-DNA complex formation and the impact of protein dimerization of the DNA-binding d...

Cytoplasmic dynein is a multisubunit ATPase that transforms chemical energy into motion along microtubules. LC8, a 10 kDa light chain subunit of the dynein complex, is highly conserved with 94% sequence identity between Drosophila and human. The precise function of this protein is unknown, but its ubiquitous expression and conservation suggest a critical role in the function of the dynein motor...

The features of midazolam (MDZ) induced structural perturbation of human serum albumin (HSA) has been studied in detail by Fourier transformation infrared spectra (FT-IR),and circular dichroism (CD).The CD spectra was deconvoluted to quantify the changes in α helix, β sheets, β turns and random coils of the protein. UV-Vis absorption studies suggested the static type quenching procedure for thi...

OBJECTIVES Aggregation of the TAU proteins in the form of neurofibrillary tangles (NFTs) in the brain is a common risk factor in tauopathies including Alzheimer's disease (AD). Several strategies have been implemented to target NFTs, among which chaperones, which facilitate the proper folding of proteins, appear to hold great promise in effectively inhibiting TAU polymerization. The aim of this...

Pomalidomide (POM), a potent anticancer thalidomide analogue was characterized in terms of cyclodextrin complexation to improve its aqueous solubility and maintain anti-angiogenic activity. The most promising derivatives were selected by phase-solubility studies. From the investigated nine cyclodextrins – differing cavity size, nature substituents, degree substitution charge highest increase ob...

Proteins adsorbed on nanoparticles (NPs) are being used in biotechnology, biosensors and drug delivery. However, understanding the effect of NPs on the structure of proteins is still in a nascent state. In the present paper tin oxide (SnO2) NPs were synthesized by the reaction of SnCl4·5H2O in methanol via the sol-gel method and characterized by x-ray diffraction (XRD), Fourier transform infrar...

The absorption, circular dichroism, and fluorescence spectra have been measured on oxidized and reduced reaction center preparations from Rhodopseudomonas spheroides strain R-26 at 295 and 77” K. The spectra indicate that the 4 bacteriochlorophyll a and 2 bacteriopheophytin a molecules in the reaction center are contained in a single pigment complex. The absorption bands at 893, 804, and ‘760 i...

The interactions of 3-carboxyphenoxathiin with Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) have been studied by fluorescence and circular dichroism spectroscopy. The binding of 3-carboxyphenoxathiin quenches the BSA and HSA fluorescence, revealing a 1:1 interaction with a binding constant of about 10(5) M(-1). In addition, according to the synchronous fluorescence spectra of BSA an...

the effects of electromagnetic fields (emfs) radiation at the frequency of 940 mhz on the structure and function of human adult and fetal hemoglobin (hba and hbf) were studied. after extraction and purification of hba and hbf, the oxygen absorption values for exposed and unexposed hba and hbf to emf were compared. the slope of oxygen absorption curve for exposed hba was increased while that for...