A simple and rapid enzymic fluorimetric method for the determination of sulphated and non-sulphated primary bile acids in urine has been developed. Octadecyisilane-bonded silica cartridges (Sep-Pak CI8) are used for the solid-phase extraction of bile acids (BA) from urine samples. Sulphated BA are solvolysed before measurement with an improved rapid solvolysis procedure. The measurement is base...

OBJECTIVES The aim of this work was to develop and validate an HPLC method for gentamicin quantification in different types of biological samples such as animal tissues and cellular material and also in pharmaceuticals. METHODS Poly(lactide-co-glycolide) microparticles (MP) of gentamicin (PLGA 502H MP), THP-1 cells, and plasma and tissue samples of mice treated with the antibiotic either free...

The fluorimetric and microbiological methods for the determination of serum and urine cephalexin were compared with the aid of coded samples in two different laboratories. The coefficient of correlation between the two methods for serum cephalexin was 0.94. At urine cephalexin concentrations above 0.5 g/l, the coefficient of correlations between two methods was 0.83. At lower concentrations (0....

A simple fluorimetric method is described for measuring free 11-hydroxycorticoids in human plasma. Only 2 ml. of plasma is required for each estimation and the fluorescence is read in a standard direct reading fluorimeter. Six estimations can be completed in one and a half hours. It thus compares favourably with the methods in current use for estimating urinary steroids, and has the added advan...