OBJECTIVES Genetic mutations and polymorphisms have been correlated with chronic pancreatitis (CP). This study aims to investigate the association of genetic variants of cystic fibrosis transmembrane conductance regulator (CFTR) and serine protease inhibitor Kazal type 1 (SPINK-1) genes and Cathepsin B gene polymorphisms with CP and to associate genetic backgrounds with clinical phenotypes. M...

Acetyl-coenzyme A carboxylase α (ACC-alpha) is considered as the key regulatory enzyme in fatty acid biosynthesis. ACC-alpha gene is located on Caprine chromosome 11 and is polymorphic in many goat breeds. In the current study, we aimed to find possible single nucleotide polymorphisms (SNPs) in the exon 1 region of the ACC-alpha gene in Iranian Mahabadi goat breed. Genomic DNA was extracted fro...

Decreased paraoxonase-1 (PON1) activity has been associated with rheumatoid arthritis. There are two polymorphisms in serum PON1; one differs in the amino acid at position 192 (Q192R) and the other one differs at position 55 (L55M). We looked for a possible association between Q192R polymorphism and rheumatoid arthritis. The Q192R polymorphism in 88 rheumatoid arthritis patients and 78 healthy ...

acetyl-coenzyme a carboxylase α (acc-alpha) is considered as the key regulatory enzyme in fatty acid biosynthesis. acc-alpha gene is located on caprine chromosome 11 and is polymorphic in many goat breeds. in the current study, we aimed to find possible single nucleotide polymorphisms (snps) in the exon 1 region of the acc-alpha gene in iranian mahabadi goat breed. genomic dna was extracted fro...

the pituitary-specific transcription factor (pit-1) gene is a candidate gene for growth, carcass and also for milk yield traits. in dairy farm animals, the main goal of the selection is the improvement of milk yield and composition. the genes of milk proteins and hormones are excellent candidate genes for linkage analysis with quantitative trait loci (qtl) because of their biological significan...

polymorphism of the b-lactoglobulin (b-lg) gene in 101 cows belonging to the holstein herd and a superior cow, producing more than 150 kg milk/day, together with four offsprings was investigated by the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method. in the holstein herd, the alleles a and b of the b-lg gene had frequencies of 0.53 and 0.47, respectively. th...

introduction : pemphigus vulgaris (pv) is a relatively rare autoimmune disease characterized by blistering of the skin and mucosa. the main objective of this study was to investigate the possible association between the 5-httlpr polymorphism and pv in iranian patients. for this, 112 pv and 100 controls were enrolled in this study. materials and methods : in this case-control study, genomic dna ...

introduction: using addictive drugs can change the amount of neurotransmitters, especially dopamine and glutamate. glutamate has been known to trigger the relapse and tendency toward addictive drugs. the glutamate receptor ionotropic nmda type subunit 1 (grin1) contains the single- nucleotide polymorphism c1001g (rs11146020) and encodes n-methyl-d-aspartic acid (ndma) receptor subunit 1 (nr1). ...

the present study investigated the calpain gene diversity in iranian one-hump camels (120 camels) using pcr-rflp method. three genotypes aa, ag and gg were observed with the frequencies of 0.02, 0.19 and 0.78, respectively. the frequencies of a and g alleles were 0.12 and 0.88, respectively. the chi-squared test didn’t show significant deviations from hardy-weinberg equilibrium (p<0.05). associ...

PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of vario...