PURPOSE To design a simple, cost-effective system for gaining rapid and accurate calyceal access during percutaneous nephrolithotomy (PCNL). MATERIALS AND METHODS The design consists of a low-cost, light-weight, portable mechanical gantry with a needle guiding device. Using C-arm fluoroscopy, two images of the contrast-filled renal collecting system are obtained: at 0-degrees (perpendicular t...

Familial hemiplegic migraine type-1 FHM-1 is caused by missense mutations in the CACNA1A gene that encodes the alpha(1A) pore-forming subunit of Ca(V)2.1 Ca(2+) channels. We used knock-in (KI) transgenic mice harboring the pathogenic FHM-1 mutation R192Q to study neurotransmission at the calyx of Held synapse and cortical layer 2/3 pyramidal cells (PCs). Using whole cell patch-clamp recordings ...

The calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium imaging in brain slices, we demonstrate that these collaterals are involved in synaptic transmission. We show ...

abstract to investigate the effects of paclobutrazol (pbz) on vegetative and reproductive growth of strawberry cv. selva a factorial experiment in a completely randomized design with three replications was carried out in greenhouse of agriculture college, isfahan university of technology. pbz was applied in two methods, drench and foliar spray, at 0, 0.5, 1, 1.5 and 2 mg active ingredient per p...

0100-3984 © Colégio Brasileiro de Radiologia e Diagnóstico por Imagem the authors have found only one case of urinary ascites secondary to rupture of a renal calyx in a newborn with posterior urethral valve. In the above mentioned case, the diagnosis of such complication was achieved by means of micturating cystourethrography. The authors describe a case of urinary ascites secondary to renal ca...

A newly recorded family, Phrymaceae, and species, Phryma leptostachya L., from Taiwan are described and illustrated. Phryma leptostachya was found near Chienshih Hsiang, in Hsinchu Hsien. The flowers of Phryma have unusually long hooked tips on the calyx and single-seeded fruit enclosed by the persistent calyx, which accounts for its isolated taxonomic position and recognition as an independent...

The large glutamatergic calyx of Held synapse in the auditory brainstem has become a powerful model for studying transmitter release mechanisms, but the molecular bases of presynaptic function at this synapse are not well known. Here, we have used single-cell quantitative PCR (qPCR) to study the developmental expression of all major Synaptotagmin (Syt) isoforms in putative calyx of Held-generat...

We developed a rodent crista slice to investigate regional variations in electrophysiological properties of vestibular afferent terminals. Thin transverse slices of the gerbil crista ampullaris were made and electrical properties of calyx terminals in central zones (CZ) and peripheral zones (PZ) compared with whole cell patch clamp. Spontaneous action potential firing was observed in 25% of cur...

The Drosophila mushroom bodies are critical association areas whose role in olfactory associative learning has been well characterized. Recent behavioral studies using a taste association paradigm revealed that gustatory conditioning also requires the mushroom bodies (Masek and Scott, 2010; Keene and Masek, 2012). Here, we examine the representations of tastes and the neural sites for taste ass...

Present data support the conclusion that protons serve as an important neurotransmitter to convey excitatory stimuli from inner ear type I vestibular hair cells to postsynaptic calyx nerve terminals. Time-resolved pH imaging revealed stimulus-evoked extrusion of protons from hair cells and a subsequent buildup of [H(+)] within the confined chalice-shaped synaptic cleft (ΔpH ∼ -0.2). Whole-cell ...