BACKGROUND High-resolution melting of PCR products is an efficient and analytically sensitive method to scan for sequence variation, but detected variants must still be identified. Snapback primer genotyping uses a 5' primer tail complementary to its own extension product to genotype the resulting hairpin via melting. If the 2 methods were combined to analyze the same PCR product, the residual ...

BACKGROUND Rotavirus infections are a major cause of diarrhea in children in both developed and developing countries. Rotavirus genetics, patient immunity, and environmental factors are thought to be related to the severity of acute diarrhea due to rotavirus in infants and young children. The objective of this study was to provide a correlation between rotavirus genotypes, clinical factors and ...

This study evaluated the frequency of the tumor necrosis factor-alpha (TNF-alpha) -308 G/A polymorphism in Brazilians with periodontal health (PH = 51), chronic periodontitis (CP = 74) and generalized aggressive periodontitis (GAgP = 38). Human DNA was obtained from mouthwash samples and TNF-alpha genotyping was performed by PCR and RFLP analyses. Differences in clinical and genetic parameters ...

The patient’s serum -1 globulins were reduced at 1.3 g/L (reference interval, 2.1–3.5 g/L). Potential causes include malnutrition, excessive excretion, decreased production, and 1-antitrypsin (A1AT) deficiency. The clinical history was consistent with A1AT deficiency (1–3 ). The diagnosis should be confirmed by direct quantification of circulating A1AT by immunoassay and confirmation of the pre...

UNLABELLED Commercially available HIV-1 drug resistance (HIVDR) genotyping assays are expensive and have limitations in detecting non-B subtypes and circulating recombinant forms that are co-circulating in resource-limited settings (RLS). This study aimed to optimize a low cost and broadly sensitive in-house assay in detecting HIVDR mutations in the protease (PR) and reverse transcriptase (RT) ...

Approximately 3% of the world population is chronically infected with the hepatitis C virus (HCV), with potential development of cirrhosis and hepatocellular carcinoma. Despite the availability of new antiviral agents, treatment remains suboptimal. Genome-wide association studies (GWAS) identified rs12979860, a polymorphism nearby IL28B, as an important predictor of HCV clearance. We report the...

BACKGROUND The TP53 rs1042522 polymorphism (c.215C>G, Arg72Pro) has been extensively investigated as a potential risk factor for colorectal cancer, but the results have thus far been inconclusive. METHODS We searched multiple electronic databases to identify studies investigating the association between the Arg72Pro polymorphism and colorectal cancer. Individual study odds ratios (OR) and the...

Abstract. As an excellent local sheep breed in China, Hu have the characteristics of producing more lambs and good motherhood. The purpose this study was to identify polymorphism mitogen-activated protein kinase 5 (MAP3K5) gene determine whether it associated with body size traits (body height, length, chest circumference, cannon circumference) sheep. MAP3K5 identified by using PCR amplificatio...

Limited genotyping data are available for rotavirus strains in the Middle East. In this study, we investigated the molecular epidemiology of human rotavirus strains circulating in the Sultanate of Oman during 2005. Rotavirus was detected in 178 (57.4%) of 310 of the diarrheal stools of young children <5 years admitted to hospitals and outpatients clinics. Polyacrylamide gel electrophoresis demo...