In solutions of serum heated at 100” at neutrality, the presence of small amounts of sulfhydryl reagents markedly alters the course of coagulation; iodoacetic acid strongly inhibits the formation of a coagulum, while iodoacetamide, methyl iodoacetate, and p-chloromercuribenzoate accelerate the rate at which a solid gel is obtained (1). The formation of a solid onephase coagulum or gel in a heat...

Methods We conducted an observational, retrospective study in two academic trauma centers from a French university institution. All patients diagnosed with HF on rotational thromboelastometry (ROTEM) between 2010 and 2014 were included. Demographic and clinical parameters, standard laboratory and ROTEM results were collected. HF diagnosis was established when the 60 minute clot lysis index (CLI...

Thrombelastography (TEG)/thromboelastometry (ROTEM) devices measure viscoelastic clot strength as clot amplitude (A). Transformation of clot amplitude into clot elasticity (E with TEG; CE with ROTEM) is sometimes necessary (eg, when calculating platelet component of the clot). With TEG, clot amplitude is commonly transformed into shear modulus (G; expressed in Pa or dyn/cm2) as follows: G = (50...

BACKGROUND Platelet-rich plasma is a fraction of plasma in which platelets are concentrated. It is reported to represent a source of multiple growth factors that promote tissue repair. In anticipation of the eventual testing of platelet-rich plasma in anterior cruciate ligament (ACL)-deficient patients, we examined the effect of autologous platelet-rich plasma on human ACL cell function in vitr...

In early diagnosis of typhoid fever, the emphasis has for long been on the isolation of S. typhi from blood culture. Clot culture techniques have not enjoyed the same vogue, although they are largely used in areas where the disease is endemic. Felix (1924) found that clot culture was hardly ever unsuccessful if blood culture was successful, but that the reverse was frequently true. He attribute...

were all prolonged. 16 of 24 tested relatives had the defect, which appeared to be transmitted as an autosomal dominant characteristic. The thrombin time of normal plasma was slightly inhibited by the proband's plasma. The abnormally long thrombin time of fibrinogen Cleveland II was partially corrected by addition of calcium ions. Fibrinogen Cleveland II was indistinguishable from normal fibrin...

INTRODUCTION This section covers the means, as far as we now know them, by which components of the blood plasma produce an insoluble protein meshwork, or gel, at a site of blood-vessel damage. The plasma is normally completely fluid, containing all its proteins in soluble form. When the clotting system is activated at a site of blood-vessel damage, a series of proteolytic reactions is set going...

Radiolabeled antibodies were perfused into fibrin clots and fibrinogen gels formed in vitro to assess the reactivity of selected epitopes. An antifibrinogen monoclonal antibody (MoAb) (antibody 1D4/xl-f), directed against an epitope in the A alpha-chain C-terminal region (A alpha 241-476), bound to 35% of the epitope in crosslinked fibrin clots and 37% of the same epitope in factor XIII-induced...