A preparative method for the isolation of oligosaccharide peptides from lysozyme digests of Micrococcus Zysodeiklicus cell walls is described. The method involves chromatography on Dowex 1, gel filtration on Bio-Gel P-4 columns, and chromatography on Dowex 50. Four glycopeptides designated as GP-1, GP-la, GP-2, and GP-4 were isolated and their structures investigated by chemical and enzymatic t...

With the aid of more sensitive instrumental methods it is possible to determine trace elements after separation of different fractions from soil and plant samples. Fractions of soils and sediments are obtained by extraction with different reagents, chosen in function of biological, ecological or chemical considerations. In plant samples it is possible to separate free ions and organo-mineral co...

A preparative method for the isolation of oligosaccharide peptides from lysozyme digests of Micrococcus Zysodeiklicus cell walls is described. The method involves chromatography on Dowex 1, gel filtration on Bio-Gel P-4 columns, and chromatography on Dowex 50. Four glycopeptides designated as GP-1, GP-la, GP-2, and GP-4 were isolated and their structures investigated by chemical and enzymatic t...

Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative p...

A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S-400, chromatography on DEAE-cellulose and affinity chromatography on calmodulin-agarose. From the parallel change in staining intensity and in th...

The effect of constituents of human platelets on leukocyte adherence was examined. Adherence-inhibiting factors (AIFs), which strongly inhibited neutrophil adherence to glass, were present in both cytosol and granule fractions of human platelets. On the Superose 6 gel chromatography (Pharmacia LKB Biotechnology, Uppsala, Sweden). the granular AIF was eluted as a single active peak (2,600 Kd), w...

The enzyme anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase from Serratia marcescens was purified to apparent homogeneity. The purification procedure included ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex gel filtration and hydroxyapatite chromatography. The molecular weight of the native protein as determined on a calibrated Sephadex G-200 column w...

A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv "White Cloud Bean". Both trypsin inhibitors exhibited a molecular mass of 16 kDa and reduced the activit...

Interferon production was induced in mouse Ehrlich ascites tumor cells by infection with Newcastle disease virus. The interferon produced was purified by precipitation with ammonium sulfate, chromatography on carboxymethyl-Sephadex, treatment with blue dextran and polyethylene glycol, gel filtration on Bio-Gel P-60 and Bio-Gel P-200, chromatography on phosphocellulose, isoelectric focusing, and...

1. A specific collagenase from the culture medium of rabbit synovial fibroblasts was purified by gel filtration and ion-exchange chromatography. 2. The enzyme was homogeneous on polyacrylamide-gel electrophoresis and showed only traces of contaminants when tested in gels with a non-specific antiserum. 3. Therabbit fibroblast collagenase could hydrolyse collagen both in solution and in fibrillar...