Leaf-color mutants in rice (Oryza sativa L.) are excellent models for studying chlorophyll biosynthesis and chloroplast development. In this study, a yellow-green-leaf mutant generated by 60Co irradiation, ygl9311, was isolated: it displayed yellow-green leaf phenotype during the complete growth cycle. Compared with wild type, photosynthetic pigment contents of leaves ygl9311 were significantly...

The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive rice pests worldwide. GXU202 a germplasm common wild (Oryza rufipogon Griff.) with high resistance to BPH. In this study, genetic analysis indicated that BPH-resistant phenotype controlled by major gene. Through combination and comparison QTL linkage BSA-seq analyses, novel gene locus, BPH41, conferring BPH was ...

: Border disease virus (BDV) is a serious pathogen of sheep and goats worldwide. The causes considerable economic losses in animal husbandry. Therefore, developing effective genetic-based therapies to control viral infections, such as BDV, has been remarkable over recent years. A common way evaluate therapeutic strategies by cloning the desired fragment into appropriate vectors develop cell lin...

BACKGROUND Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinder successful generation of overexpression constructs if these sequences are also present in the target cDNA insert. RESULTS Utilizing ligation-independ...

background: bovine viral diarrhea virus (bvdv) causes significant economic and health effects in cattle farms. because of the relative inefficiency of bvdv eradication programs, in recent years, numerous strategies such as anti-viral gene therapy has been used extensively to fight it. one of the ways to evaluate the quality of these anti-viral strategies is the study of their efficacy in the sp...

Rapid and efficient construction of expression vectors and subsequent transformation are basic recombinant methods for the investigation of gene functionality. Although novel cloning methods have recently been developed, many laboratories worldwide continue to use traditional restriction digestion-ligation methods to construct expression vectors owing to financial constraints and the unavailabi...

Bacterial-based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. Developments in genetic engineering have given Gram-positive lacticacid bacteria (LAB) the advantage of being used as a host expression system for antigen delivery to inducethe immune response. A fragment containing the full length of the “eprA1” ...