Cancerous transformation may be dependent on correlation between electrical disruptions in the cell membrane and mechanical disruptions of cytoskeleton structures. Silicon nanotube (SiNT)-based electrical probes, as ultra-accurate signal recorders with subcellular resolution, may create many opportunities for fundamental biological research and biomedical applications. Here, we used this techno...

Experimental detachments were made in Dutch rabbit eyes by injecting autologous serum or Hanks' solution into the subretinal space through a glass micropipette. The serum was resorbed at a rate of 0.064 +/- 0.023 microliter/mm2/hr, which is approximately half as fast as Hanks' solution. Both cyclic GMP and dibutyryl cyclic GMP accelerated serum resorption by 23%, whereas cyclic AMP and dibutyry...

The local ERG (LERG) from detached retina within the intact rabbit eye has been recorded. Retinal detachments were made in Dutch rabbit eyes by injecting Hanks' balanced salt solution into the subretinal space through a glass micropipette. Electrical responses were recorded with a double-barrelled microelectrode that was advanced through the vitreous until the tip penetrated the retina to enter...

The function of the glymphatic system was evaluated by T1-weighted MRI after intracisternal DOTA-Gd (Dotarem, Guerbet, France) microinjection (1 μL for 1 minute). We modified a previously reported method by using a pulled hematological glass micropipette (Figure I in the online-only Data Supplement). Regions of interest for subsequent signal analyses were selected as shown in Figure II in the o...

Pulmonary capillary hemangiomatosis is a rare cause of pulmonary hypertension characterized by extensive proliferation of pulmonary capillaries within alveolar septae. Clinical presentation is nonspecific and includes dyspnea, cough, chest pain, and fatigue. Radiology shows diffuse centrilobular ground-glass opacities. Pulmonary capillary hemangiomatosis is clinically and radiographically indis...

Abstract Our previous study has revealed that acoustic resonances have non-negligible effects on photoacoustic signals for a liquid sample confined in thin tube. In this paper, we present numerical and experimental studies the of tube hardness directivity generated signal. A glass capillary silicone 1 mm diameter were tested using pulsed light 637 nm wavelength. Black ink was as material. capil...

Hollow glass tubes are in use at Cornell High Energy Synchrotron Source (CHESS) as X-ray optics in the 4 to 25 keV range to take large diameter, fairly parallel X-ray beams and compress them into smaller diameter, more divergent beams for various kinds of X-ray applications. The optics come in two forms: 1) multibounce condensing capillaries for making submicron diameter beams and 2) one-bounce...

A high aspect ratio 3D electrokinetic nanoprobe is used to trap polystyrene particles (200 nm), gold nanoshells (120 nm), and gold nanoparticles (mean diameter 35 nm) at low voltages (<1 V(rms)). The nanoprobe is fabricated using room temperature self-assembly methods, without the need for nanoresolution lithography. The nanoprobe (150-500 nm in diameter, 2-150 μm in length) is mounted on the e...

Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in reasonable periods of time. A novel system that will rapidly load, clean, and accurately position ...

This paper presents processes for glass micromachining, including sandblast, wet etching, reactive ion etching (RIE), and glass reflow techniques. The advantages as well as disadvantages of each method are presented and discussed in light of the experiments. Sandblast and wet etching techniques are simple processes but face difficulties in small and high-aspect-ratio structures. A sandblasted 2...