BACKGROUND β-Glucanase is one of the most extensively used biocatalysts in biofuel, food and animal feed industries. However, the poor thermostability and low catalytic efficiency of most reported β-glucanases limit their applications. Currently, two strategies are used to overcome these bottlenecks, i.e., mining for novel enzymes from extremophiles and engineering existing enzymes. RESULTS A...

The biological control agent Pseudomonas fluorescens was used against the bacterial wilt causing Ralstonia solanacearum. The present investigation focuses on the role of defense related enzymes in imparting resistance in tomato against R. solanacearum. A total of ten rhizobacterial isolates were screened against R. solanacearum, of which three isolates (Pf3, Pf5 & Pf8) showed a maximum inhibiti...

The β-glucanase enzyme is an protein that can hydrolyze β-glucan, one of the main components fungal cell wall. This produced by several bacteria, which B. subtilis. three-dimensional (3D) structure proteins necessary to understand their properties and functions proteins. Enzyme be analyzed for function using in silico method. study aims detect gene from subtilis W3.15 analyze it methods this re...

An experiment was conducted to determine the effect of graded levels of β-glucanase supplementation to barley (Hordeum vulgare)-based diets on the digestibility of DM, GE, N, and AA for growing-finishing pigs. Eight pigs (initial BW: 53.3 ± 3.2 kg) were each fitted with a simple T-cannula at the distal ileum and allotted to a replicated 4 × 4 Latin square design with 4 diets and 4 periods in ea...

Beta-1,3-glucanases are enzymes that hydrolyze beta-1,3-glucans, and they essential for the metabolism of seaweed, plants fungi. These also participate in digestion herbivore fungivore animals. Because importance these enzymes in insects, beta-1,3-glucanase inhibitors may be used development new control strategies against agricultural pests disease vectors. Beta-1,3-glucanase inhibito...

1,3-1,4-β-glucanase is an important biocatalyst in brewing industry and animal feed industry, while its low thermostability often reduces its application performance. In this study, the thermostability of a mesophilic β-glucanase from Bacillus terquilensis was enhanced by rational design and engineering of disulfide bonds in the protein structure. Protein spatial configuration was analyzed to p...

Cell suspension cultures of chickpea (Cicer arietinum L.) were used to prepare protoplasts and vacuoles. The vacuolar preparation revealed only slight contaminations of cytoplasmic marker enzymes. HPLC analysis of the vacuolar extract showed that the malonylglucosides of isoflavones, isoflavanones and pterocarpans are exclusively located in the vacuole. Experi­ ments designed to determine the s...

The mutant E134A 1,3-1,4-beta-glucanase from Bacillus licheniformis, in which the catalytic nucleophilic residue has been removed by mutation to alanine, has its hydrolytic activity rescued by exogenous formate in a concentration-dependent manner. A long-lived alpha-glycosyl formate is detected and identified by (1)H-NMR and matrix-assisted laser desorption ionization-time-of-flight-MS. The int...

BACKGROUND Enzymes for plant cell wall deconstruction are a major cost in the production of ethanol from lignocellulosic biomass. The goal of this research was to develop optimized synthetic mixtures of enzymes for multiple pretreatment/substrate combinations using our high-throughput biomass digestion platform, GENPLAT, which combines robotic liquid handling, statistical experimental design an...