Bakground: Aspergillus fumigatus is an airborne opportunistic fungal pathogen that can cause fatal infections in immunocompromised patients. Although the current anti-fungal therapies are relatively efficient, some issues such as drug toxicity, drug interactions, and the emergence of drug-resistant fungi have promoted the intense research toward finding the novel drug targets. Methods: In searc...

2. Ribose from each nucleotide has been degraded and the distribution of isotope has been determined. 3. The distribution of isotope suggests that oxidation and decarboxylation of glucose 6phosphate is largely responsible for synthesis of ribose. 4. Other reactions that contribute to ribose synthesis probably include those catalysed by transaldolase, transketolase, aldolase and triose phosphate...

Whether bone marrow stromal cells of donors contribute physiologically to hematopoietic stem cell reconstitution after marrow transplantation is unknown. To determine the transplantability of nonhematopoietic marrow stromal cells, stable clonal stromal cell line (GB1/6) expressing the a isoenzyme of glucose-6-phosphate isomerase (Glu6PI-a, D-glucose-6-phosphate ketol-isomerase; EC 5.3.1.9) was ...

Two mannose-negative mutants of Escherichia coli K-12 have been isolated. These mutants are deficient in the ability to synthesize phosphomannose isomerase and capsular polysaccharide when grown on glucose-containing media. Interrupted mating experiments to determine the kinetics of genetic transfer show that the two mannose-negative mutations map together between the histidine and tryptophan r...

The glucose analogue, 2-deoxy-n-glucose, has been found to inhibit glycolysis and the growth of yeast (1, 2) and various animal tissues (3-7). It is phosphorylated by hexokinase (8), but is not believed to be further metabolized. Wick et al. (9) have reported that deoxyglucose B-phosphate competitively inhibits the conversion of glucose 6-phosphate to fructose 6-phosphate by a purified phosphoh...

In a sustainable society based on circular economy, the use of waste lignocellulosic biomass (LB) as feedstock for biorefineries is promising solution, since LB world’s most abundant renewable and non-edible raw material. available by-product from agricultural forestry processes, its main components are cellulose, hemicellulose, lignin. Following suitable physical, enzymatic, chemical steps, di...

The use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. Development of immobilized/stabilized biocatalysts could be a further option to improve the process. In this work, β-galactosidase from Bacillus circulans, l-arabinose (d-galactose) isomerase from Enterococcus faecium, and d-xylose (d-glucose) isomerase from Str...