A number of S-substituted glutathiones and the corresponding N-substituted S-substituted analogues have been found to be linear competitive inhibitors of yeast glyoxalase I at 26 degrees C over the pH range 4.6-8.5. N-Acetylation of S-(p-bromobenzyl)glutathione weakens binding by 13.7-fold. N-benzoylation by 25.6-fold, N-trimethylacetylation by 53.3-fold and N-carbobenzoxylation by 7.8-fold, in...

Crystalline glutathione reductase, prepared from yeast (3) was a generous gift from Dr. E. Backer. The enzyme had a specific activity of 8.8 units (pmoles of TPNH oxidized per minute) per mg of protein, after lyophilization, but had lost approximately three-fourths of this activity when it was used in the experiments here described. Another preparation of glutathione reductase was obtained from...

OBJECTIVE We investigated the role of the glutathione S-transferase A1, M1, P1 and T1 gene polymorphisms and potential effect modification by occupational exposure to different chemicals in Serbian bladder cancer male patients. PATIENTS AND METHODS A hospital-based case-control study of bladder cancer in men comprised 143 histologically confirmed cases and 114 age-matched male controls. Delet...