The purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. Optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). I...

The axial skeleton is a defining feature of vertebrates and is patterned during somitogenesis. Cyclically expressed members of the notch and other signaling pathways, described as the 'segmentation clock', regulate the formation of somite boundaries. Comparisons among vertebrate model systems have revealed fundamental shifts in the regulation of expression among critical genes in the notch path...

Matter has three states or forms of aggregation: solid, liquid and gaseous. When referring to matter and its states, we imagine quantum physicists, chemists and engineers in big laboratories or in particle accelerators to which basic researchers have little or no access. The least we could imagine is physicists describing a new state of matter in the eyes of a common chicken and, what is even m...

Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions ob...

PURPOSE Combining techniques of episomal vector gene-specific Cre expression and genomic integration using the piggyBac transposon system enables studies of gene expression-specific cell lineage tracing in the chicken retina. In this work, we aimed to target the retinal horizontal cell progenitors. METHODS A 208 bp gene regulatory sequence from the chicken retinoid X receptor γgene (RXRγ208) ...

Under the shell of a chicken egg are two opposed proteinaceous disulfide-rich membranes. They are fabricated in the avian oviduct using fibers formed from proteins that are extensively coupled by irreversible lysine-derived crosslinks. The intractability of these eggshell membranes (ESM) has slowed their characterization and their protein composition remains uncertain. In this work, reductive a...